ArticleProceedings of the National Academy of Sciences of the United States of America2025
Dynamic changes in histone lysine lactylation during meiosis prophase I in mouse spermatogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- H3K18 Lactylation Promotes Cell Proliferation in T-Cell Acute Lymphoblastic Leukemia.Journal of cellular biochemistry · 2026Article
- Swimming in Plastamination: Polylactic Acid (PLA) Nanoplastics Affect Bull Sperm Functions.International journal of molecular sciences · 2026Article
- Crosstalk between lactylation and other post-translational modifications in health and diseases.Molecular biomedicine · 2026Review
- Lactate-fueled H3K18 lactylation sustains ΔNp63α-dependent self-renewal in limbal epithelial stem cells.The Journal of biological chemistry · 2026Article
- Both L-Lactyl and D-Lactyl Enantiomers Modify Histones in Mouse Testis.Molecular & cellular proteomics : MCP · 2026Article
- Lactylation-driven therapeutic resistance in cancer: Mechanisms and therapeutic opportunities.Genes & diseases · 2026Review
- Upregulation of mScience China. Life sciences · 2026Article
- MCT1 governs a metabolic checkpoint at pachytene during spermatogenesis.Research square · 2026Article
- MCT1 governs a metabolic checkpoint at pachytene during spermatogenesis.bioRxiv : the preprint server for biology · 2026Article
- Review
- A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice.Nucleic acids research · 2026Article
- Lysine lactylation in diseases: beyond histone lactylation.Cell death & disease · 2025Review
- Genetic and epigenetic landscape of male infertility.Trends in genetics : TIG · 2025Review
- Class I histone deacetylases catalyze lysine lactylation.The Journal of biological chemistry · 2025Article
- L-2-hydroxyglutarate regulates centromere and heterochromatin conformation in the male germline.PLoS genetics · 2025Article
- Dynamic changes in histone lysine lactylation during meiosis prophase I in mouse spermatogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Histone Lactylation in Diseases: Regulation by Traditional Chinese Medicine and Therapeutic Implications.Drug design, development and therapy · 2025Review
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3 authors.
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Abstract
Male germ cells, which are responsible for producing millions of genetically diverse sperm through meiosis in the testis, rely on lactate as their central energy metabolite. Recent study has revealed that lactate induces epigenetic modification in cells through histone lysine lactylation. Here, we report dynamic histone lactylation at histone H4-lysine 5 (K5), -K8, and -K12 during meiosis prophase I in mouse spermatogenesis. By profiling the genome-wide occupancy of histone H4-K8 lactylation (H4K8la), which peaks at zygotene, our data show that H4K8la mark is observed at the promoters of genes exhibiting active expression with Gene Ontology functions enriched for meiosis. Notably, our data also demonstrate that H4K8la is closely associated with recombination hotspots, where machinery involved in the processing DNA double-stranded breaks, such as SPO11, DMC1, RAD51, and RPA2, is engaged. In addition, H4K8la was also detected at the meiosis-specific cohesion sites (marked by RAD21L and REC8) flanking the recombination hotspots. Functionally, our data show that lactate induces upregulation of key meiotic genes through H4K8la modifications. Additionally, H4K8la shows colocalization and interaction with PRDM9 at recombination hotspots. Finally, our data show that HBO1, a lactyltransferase, is highly expressed in meiotic germ cells. In vitro lactylation assays reveal that HBO1 induces H4K8la, and pharmacological inhibition of HBO1 in mice reduces H4K8la levels and disrupts meiosis. Collectively, our findings suggest that histone lactylation serves as an epigenetic mechanism that is involved in meiotic gene expression and recombination in male germ cells during spermatogenesis.
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