Evidence map›Paper›PMID 39927766›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Customization of Ethylene Glycol (EG)-Induced BmoR-Based Biosensor for the Directed Evolution of PET Degrading Enzymes.

Min Li, Zhenya Chen, Wuyuan Zhang, Tong Wu, Qingsheng Qi, Yi-Xin Huo

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Min LiDepartment of Gastroenterology, Aerospace Center Hospital, College of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.ORCID https://orcid.org/0009-0005-7707-2314
Zhenya ChenDepartment of Gastroenterology, Aerospace Center Hospital, College of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.ORCID https://orcid.org/0000-0002-5900-8547
Wuyuan ZhangDepartment of Gastroenterology, Aerospace Center Hospital, College of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.
Tong WuDepartment of Gastroenterology, Aerospace Center Hospital, College of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.
Qingsheng QiState Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.
Yi-Xin HuoDepartment of Gastroenterology, Aerospace Center Hospital, College of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Haidian District, Beijing, 100081, China.ORCID https://orcid.org/0000-0003-4644-999X

Funding

National Natural Science Foundation of China 22278033National Natural Science Foundation of China 31961133014Natural Science Foundation of Hebei Province B2023105008Tangshan Science and Technology Bureau 23130228E
6 · The paper itself

Abstract

The immense volume of plastic waste poses continuous threats to the ecosystem and human health. Despite substantial efforts to enhance the catalytic activity, robustness, expression, and tolerance of plastic-degrading enzymes, the lack of high-throughput screening (HTS) tools hinders efficient enzyme engineering for industrial applications. Herein, we develop a novel fluorescence-based HTS tool for evolving polyethylene terephthalate (PET) degrading enzymes by constructing an engineered BmoR-based biosensor targeting the PET breakdown product, ethylene glycol (EG). The EG-responsive biosensors, with notably enhanced dynamic range and operation range, are customized by fluorescence-activated cell sorting (FACS)-assisted transcription factor engineering. The ingeniously designed SUMO-MHETase-FastPETase (SMF) chimera successfully addresses the functional soluble expression of MHETase in Escherichia coli and mitigates the inhibitory effect of mono-(2-hydroxyethyl) terephthalic acid (MHET) intermediate commonly observed with PETase alone. The obtained SM

Indexed as

Biosensing TechniquesDirected Molecular EvolutionEthylene GlycolPolyethylene TerephthalatesEscherichia coliHigh-Throughput Screening AssaysEthylene GlycolPolyethylene Terephthalatesbiosensorsdirected evolutionhigh‐throughput screeningPET biodegradationvalue‐added bioconversion

Identifiers

PMID39927766
PMCPMC11967783

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.