ArticleOpen veterinary journal2024
Isolation and PCR-based detection of parvovirus in dogs.
Article in Open veterinary journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Antiulcer activity ofOpen veterinary journal · 2025Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The Aim: This study was performed to identify the Methods: Sixty fecal samples were taken from suspected infected dogs in the Baghdad Governorates who were clinically sick and thought to have CPV. The first performed qualitative antigen detection on the samples with a rapid test, then the positive samples were propagated on the feline kidney (FK) and embryonated chicken fibroblast cells and finally identified the isolated virus on six randomly selected samples by amplifying the VP2 gene with a conventional polymerase chain reaction. Results: Rapid tests found 50% of fecal swabs as positive results. Additionally, the results of the cytopathic effect (CPE) at the second and third passages on chicken embryo and FK cell cultures revealed rounder and more detached cells. Furthermore, the isolated virus was confirmed through amplified the Vp2 gene of the virus by polymerase chain reaction. Conclusion: The focus of this study was to isolate of parvovirus that causes diarrhea in puppies, which is quite frequent in Iraq. The virus was isolated using both primary and secondary cell cultures. The solitary virus causes cells to become more differentiated and rounded due to CPEs. The virus was confirmed to be real using a polymerase chain reaction experiment. This study also suggests doing more epidemiological research and sequencing analysis to determine the prevalence of CPV serotypes in Iraq and the efficacy of trade in and locally made vaccinations in preventing against viral infection.
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