Combination Fractional Carbon Dioxide Laser Treatment and Bone Marrow Mesenchymal Stem Cell Therapy Enhances the Treatment of Skin Photoaging in a Murine Model System.

Background: Fractional carbon dioxide lasers and bone marrow mesenchymal stem cells (BMSCs) are commonly employed in the treatment of skin photoaging. Objective: This study was developed to explore the effects of combination carbon dioxide laser treatment and BMSC injection on skin photoaging and the underlying molecular mechanisms. Methods & Materials: In total, 24 mice with experimentally photoaged skin were separated into control, carbon dioxide fractional laser treatment, combination therapy, and BMSC injection groups. Samples of dorsal skin from these animals were subjected to hematoxylin and eosin staining or Masson's trichrome staining. In addition, immunohistochemical analyses and real-time polymerase chain reaction analyses were conducted to detect MMP-3 and MMP-9 expression. Results: After 1 week, both dermal thickness and collagen fiber density were significantly increased in the BMSC and combination treatment groups as compared to the control group ( Conclusion: Combination BMSC and carbon dioxide laser therapy was more effective than either of these therapeutic approaches in isolation as a treatment for photoaged skin. The improvement of effect may be due to the decrease of MMP-3 and MMP-9 expression in combination therapy.