ArticleOpen life sciences2025
Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification.
Article in Open life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Integrated Transcriptomic and Metabolomic Analyses Reveal Acyl-CoA Dehydrogenase-Mediated Primordium Formation and Metabolite Synthesis inJournal of fungi (Basel, Switzerland) · 2026Article
- Whole-Genome Resequencing Reveals Genetic Variation and Selection Signals inJournal of fungi (Basel, Switzerland) · 2026Article
- Genome-wide exploration of biosynthetic gene clusters and their association with virulence in the entomopathogenic fungus Beauveria.Functional & integrative genomics · 2026Article
- DNA barcoding and phylogenetic insights into the selected endemic flora of the Western Himalayas.Scientific reports · 2026Article
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- An optimized DNA extraction protocol for reliable PCR-based detection and characterization of grapevine flavescence dorée phytoplasma.Plant methods · 2025Article
- Biocontrol Potential of Rhizosphere Bacteria AgainstBiology · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Genomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the target gene and minimize deoxyribonucleic acid (DNA) degradation. The modified cetyltrimethylammonium bromide (CTAB) method was found to be effective in releasing high molecular weight gDNA with minimal protein contamination. Based on anticipated gDNA yield and quality, extraction time, cost effectiveness, successful amplification, and waste management, our findings serve as a guide for selecting techniques of gDNA extraction from fungal species. This study presents a modified CTAB method for extracting DNA from a variety of fungal species including
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