ArticleIranian journal of veterinary research2024
Cloning and expression of Fusion and Hemagglutinin proteins of peste des petits ruminants virus in the baculovirus system: an immunogenicity study in mice.
Article in Iranian journal of veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Aims: The present study aimed to clone and express F and H genes in baculovirus, and evaluate the immunogenicity of recombinant proteins produced by sf9 cells in mice. Methods: Amplified F and H genes (by RT-PCR using specific primers) were cloned into pFastBac Dual plasmid. The recombinant plasmid was transformed in DH10Bac host cells. SDS-PAGE and Western blotting was performed to control the recombinant protein, and a whole pure and specific recombinant protein was obtained. Results: The immunogenicity of 20 μg of non-adjuvant recombinant proteins in Balb-c mice showed better results compared with the attenuated PPR vaccine. Conclusion: The recombinant protein obtained from this study can be a suitable candidate for the production of recombinant vaccines against PPRV.
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