ArticleRapid communications in mass spectrometry : RCM2025
A Novel Method to Determine the Carbon Isotopic Composition of Inositol Hexaphosphate (Phytate) in Soil by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry.
Article in Rapid communications in mass spectrometry : RCM, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Microbial phosphorus cycling in terrestrial ecosystems.Nature reviews. Microbiology · 2026Review
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Authors and funding
2 authors.
Funding
Abstract
rationaleUnderstanding the decomposition of inositol hexaphosphate (phytate), the dominant form of organic phosphorus (OP) in soil, is vital for studying phosphorus (P) cycling in terrestrial ecosystems. However, the lack of multiple stable P isotopes complicates the study of phytate dynamics under natural conditions and over long periods.
methodsA novel method is presented to determine the carbon isotopic composition of inositol in phytate using compound-specific isotope analysis. For this purpose, phytate was extracted from soil and purified via ion exchange chromatography, followed by dephosphorylation, derivatization, and analysis using GC-MS and GC-C-IRMS. Pure compounds were also analyzed to assess protocol efficiency, identify isotopic fractionations, and apply isotopic corrections due to derivatization.
resultsPhytate extracted from soil samples was identified using GC-MS chromatograms. Replicate analyses of the pure compounds indicated that the protocol is highly reproducible. The carbon isotopic composition (δ
conclusionsThis study offers a novel approach of determining δ
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