Evidence map›Paper›PMID 39924799›Full record

ArticleScandinavian journal of immunology2025

Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.

Abbas Habibalahi, Ayad G Anwer, Aline Knab, Shane T Grey, Ewa M Goldys, Jared M Campbell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scandinavian journal of immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Abbas HabibalahiGraduate School of Biomedical Engineering, Faculty of Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Ayad G AnwerGraduate School of Biomedical Engineering, Faculty of Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Aline KnabGraduate School of Biomedical Engineering, Faculty of Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Shane T GreyTransplantation Immunology Laboratory, Garvan Institute of Medical Research, Darlinghurst, New South Wales, Australia.
Ewa M GoldysGraduate School of Biomedical Engineering, Faculty of Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Jared M CampbellGraduate School of Biomedical Engineering, Faculty of Engineering, University of New South Wales, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0003-0163-4251

Funding

Australian Research Council DP210102960Cancer Council NSW 2021/ECF1291
6 · The paper itself

Abstract

Evaluating immune status is a challenging and time-consuming process that involves analysing various biomarkers through numerous assays. The sensitive label-free technique of multispectral imaging of cell autofluorescence involves directly assessing the molecular composition of cells to gather biological information. Cells were cultured in RPMI 1640 modified media supplemented with penicillin-streptomycin and 10% foetal bovine serum at 37°C, with 5% CO

Indexed as

B-LymphocytesMacrophagesOptical ImagingT-LymphocytesCell DifferentiationHL-60 CellsHumansJurkat CellsMonocytesNeutrophilsTHP-1 Cellsautofluorescencecytometryhyperspectralimmunemultispectral

Identifiers

PMID39924799
PMCPMC11808199

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.