Evidence map›Paper›PMID 39924789›Full record

ReviewProteomics2025

Key Considerations on CITE-Seq for Single-Cell Multiomics.

Hye-Wong Song, Jody Martin, Xiaoshan Shi, Aaron J Tyznik

Abstract readReview
In one paragraph

Review in Proteomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
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  9. Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Hye-Wong SongSingle-Cell Multiomics Team, BD Biosciences, San Diego, California, USA.
Jody MartinSingle-Cell Multiomics Team, BD Biosciences, San Diego, California, USA.
Xiaoshan ShiApplied Research & Technology, Medical Scientific Affairs, BD Biosciences, Milpitas, California, USA.
Aaron J TyznikApplied Research & Technology, Medical Scientific Affairs, BD Biosciences, San Diego, California, USA.ORCID 0000-0002-9120-8530

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is an advanced single-cell sequencing method to profile both gene expression and protein abundance simultaneously in individual cells using single-cell mRNA sequencing techniques alongside antibody-derived tags (ADTs), for protein detection. The characterization of both the transcriptome and the proteome from the same cells provides a powerful and multiomic approach for understanding the mechanisms of complex biological processes. This review focuses on the workflow of CITE-Seq using a microwell-based single-cell analysis system as an example and provides key considerations for staining cells with ADTs. By highlighting critical information for CITE-Seq library preparation, sequencing, and data analysis, this review provides a practical guide with which to perform comprehensive CITE-Seq workflow.

Indexed as

Gene Expression ProfilingProteomicsSequence Analysis, RNASingle-Cell AnalysisTranscriptomeAnimalsEpitopesHigh-Throughput Nucleotide SequencingHumansMultiomicsProteomeEpitopesProteomeantibody‐derived tagscellular indexing of transcriptomes and epitopes by sequencinglibrary preparationnext‐generation sequencingproteogenomics

Identifiers

PMID39924789
PMCPMC12645808

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.