ArticleAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica2025
The EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing of Oral Anaerobes.
Article in APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
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- When Culture Results Don't Tell the Whole Story: A Practical Review of Antibiotics Pitfalls for Clinical Decision-Making.Infectious diseases and therapy · 2026Review
- Characterization of Bacillus atrophaeus Strain from the Composite Material Surface in Arctic Conditions and Its Sensitivity to CuO Nanoparticles.Current microbiology · 2026Article
- In Vitro Antimicrobial Potential of Different Platelet Concentrates Against Eight Clinically Relevant Oral Pathobionts.Antibiotics (Basel, Switzerland) · 2026Article
- A Two-Year Study on Swifts (Pathogens (Basel, Switzerland) · 2026Article
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- The Effect of Sub-Minimal Inhibitory Concentrations of Daptomycin and Linezolid on Biofilm Formation of Methicillin ResistantTurkish journal of pharmaceutical sciences · 2025Article
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- Phage-Encoded Antimicrobial Peptide gp28 Demonstrates LL-37-Like Antimicrobial Activity Against Multidrug-ResistantPHAGE (New Rochelle, N.Y.) · 2025Article
- Amoxicillin-metronidazole disk for antimicrobial susceptibility testing of subgingival bacteria.Journal of oral microbiology · 2025Article
- Investigations of microbial adaptation to singular, binary, and fully formulated quaternary ammonium compounds.Applied and environmental microbiology · 2024Article
- Sliding motility of Bacillus cereus mediates vancomycin pseudo-resistance during antimicrobial susceptibility testing.The Journal of antimicrobial chemotherapy · 2024Article
- The Home Environment Is a Reservoir for Methicillin-Resistant Coagulase-Negative Staphylococci and Mammaliicocci.Antibiotics (Basel, Switzerland) · 2024Article
- Prevalence of piperacillin/tazobactam resistance in invasiveJAC-antimicrobial resistance · 2024Article
- Article
- Carriage of β-lactamase and carbapenemase-producingHeliyon · 2023Article
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4 authors.
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Abstract
There is a need for standardized methods for antimicrobial susceptibility testing (AST) of anaerobic bacteria involved in oral and extra-oral infections. We tested the recently published EUCAST disk diffusion method for rapidly growing anaerobes on selected oral anaerobes. AST of 20 strains of Prevotella spp., 11 strains of Porphyromonas gingivalis, and six Fusobacterium nucleatum complex strains was performed with amoxicillin and metronidazole disks using EUCAST guidelines. Plates were incubated anaerobically, and inhibition zones were evaluated after 20 h (EUCAST recommendations) and again after 44 h. The recommended agar supported the growth of all 38 strains. Twenty-hour incubation was sufficient for the assessment of inhibition zone diameters of Fusobacterium strains. Although approved for Prevotella, an extended study of Prevotella species showed inconsistent growth within the EUCAST time limit of 20 h for some strains. All P. gingivalis strains required 44 h of incubation for the evaluation of inhibition zones. The EUCAST disk diffusion method for AST of rapidly growing anaerobes is applicable to members of the Fusobacterium nucleatum complex. P. gingivalis and several oral strains of Prevotella needed 44 h of incubation to enable reading of diffusion diameter. Further studies are necessary to validate the prolonged incubation of slow-growing anaerobes.
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