Evidence map›Paper›PMID 39923455›Full record

ArticlePoultry science2025

Integrating whole-genome re-sequencing and transcriptome data to reveal the molecular mechanism of TBX5 gene regulating feathered feet in Guangxi native chickens.

Zhuliang Yang, Yongcui Liu, Jintang Luo, Cong Xiao, Hong Yuan, Wenya Du, Xiurong Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zhuliang YangCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China; College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Yongcui LiuCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Jintang LuoCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Cong XiaoCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Hong YuanCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Wenya DuCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Xiurong YangCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning 530004, China. Electronic address: yangxiurong@gxu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feathered foot is a trait observed in domestic and wild chickens, resulting from the partial or complete transformation of scales on the tarsus, shanks, and toes into feathers. Although previous studies have mapped genomic loci associated with feathered feet, the molecular mechanisms underlying this transformation remain unclear. This study combined whole-genome re-sequencing (WGS) data, transcriptomic analysis, and cellular experiments to investigate the genetic basis of feathered feet in Guangxi native chickens. A genome-wide association study (GWAS) involving 1,735 Guangxi native chickens identified two genomic regions and several candidate genes, including TBX3, TBX5, and H2AFY. RNA sequencing (RNA-seq) and real-time fluorescent quantitative PCR (QRT-PCR) results confirmed that TBX5 is differentially expressed between chickens with scaled and feathered feet, indicating its role as a key candidate gene for feathered feet. The function of TBX5 was subsequently explored at the cellular level using chicken dermal fibroblasts. The results showed that moderate overexpression of TBX5 (20 ng) significantly increased the expression of cell proliferation-related genes. In contrast, excessive overexpression of TBX5 (2.5 μg) and interference with TBX5 inhibited these genes, suggesting a dose-dependent effect of TBX5 on the proliferation of dermal fibroblasts. EdU fluorescence staining, flow cytometry, and migration assays demonstrated that moderate expression of TBX5 can promote the proliferation and migration of dermal fibroblasts, while TBX5 interference produced opposite results. Our findings suggest that TBX5 is a pivotal candidate gene associated with feathered feet in Guangxi native chickens. It is proposed that TBX5 may affect the formation of feathered feet by regulating the proliferation and migration of dermal fibroblasts.

Indexed as

Avian ProteinsChickensT-Box Domain ProteinsTranscriptomeAnimalsChinaGene Expression ProfilingGenome-Wide Association StudyWhole Genome SequencingAvian ProteinsT-Box Domain ProteinsT-box transcription factor 5Cell proliferation and migrationDermal fibroblastFeathered feetGuangxi native chickenTBX5

Identifiers

PMID39923455
PMCPMC12011099

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.