Evidence map›Paper›PMID 39923238›Full record

ArticleCell reports2025

Distinct plasmablast developmental intermediates produce graded expression of IgM secretory transcripts.

Evelyn P Sievert, Marissa C Franke, Kayla B Thomas, Yoseop Yoon, Yongsheng Shi, Roger Sciammas

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Metabolism of tumor infiltrating T cells.Frontiers in immunology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Evelyn P SievertDepartment of Anatomy, Physiology, and Cell Biology, University of California at Davis, Davis, CA, USA.
Marissa C FrankeDepartment of Anatomy, Physiology, and Cell Biology, University of California at Davis, Davis, CA, USA.
Kayla B ThomasDepartment of Anatomy, Physiology, and Cell Biology, University of California at Davis, Davis, CA, USA.
Yoseop YoonDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California at Irvine, Irvine, CA, USA.
Yongsheng ShiDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California at Irvine, Irvine, CA, USA. Electronic address: yongshes@uci.edu.
Roger SciammasDepartment of Anatomy, Physiology, and Cell Biology, University of California at Davis, Davis, CA, USA. Electronic address: rsciammas@ucdavis.edu.

Funding

National Institute on Aging (NIA) ColonyP51OD011107 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Simon J. Atkinson · 2012 to 2026
$191.5M
mRNA alternative polyadenylation in B cell developmentR01AI170840 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI Roger Sciammas, Yongsheng Shi · 2022 to 2026
$4.1M
Heterogeneity of plasma cell states is regulated by the dynamics of IRF4 expressionR21AI151610 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SCIAMMAS, ROGER · 2020 to 2021
$432k
NIAID NIH HHS R01 AI170840NIAID NIH HHS R21 AI151610NIH HHS P51 OD011107
6 · The paper itself

Abstract

Differentiation into plasma cells (PCs) enables secretion of ∼10,000 immunoglobulin molecules per second. This extraordinary capacity requires the upregulation of PC transcriptional determinants that specify PC fate, increase immunoglobulin mRNA synthesis, coordinate alternative 3' end processing of the heavy chain transcript from the distal to proximal polyadenylation site (PAS), and remodel the secretory pathway. We developed a dual-fluorescent protein reporter mouse to prospectively study the post-transcriptional-level transition from membrane anchored to secretory immunoglobulin M; μM-PAS and μS-PAS, respectively. We observed (1) graded μS-PAS usage during PC differentiation, (2) IRF4 and Blimp-1 functioned hierarchically to increase μ abundance as well as μS-PAS usage, and (3) graded μS populations did or did not express Blimp-1. Interestingly, the low and high μS and Blimp-1-expressing populations arose from distinct developmental intermediates that exhibited dissimilar endoplasmic reticulum features. The distinct cell and μS-PAS fate trajectories may have implications for derivatization of the secretory pathway.

Indexed as

Immunoglobulin MPlasma CellsAnimalsCell DifferentiationEndoplasmic ReticulumInterferon Regulatory Factor-4Interferon Regulatory FactorsMiceMice, Inbred C57BLPolyadenylationPositive Regulatory Domain I-Binding Factor 1RNA, MessengerImmunoglobulin MInterferon Regulatory Factor-4Interferon Regulatory FactorsPositive Regulatory Domain I-Binding Factor 1Prdm1 protein, mouseRNA, Messengersecretory IgMalternative polyadenylationantibodyB cell differentiationCP: ImmunologyIgMplasma cellsecretion

Identifiers

PMID39923238
PMCPMC12023845

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.