Evidence map›Paper›PMID 39922995›Full record

ArticleBMC pregnancy and childbirth2025

Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis.

Wenli Xu, Lu Li, Hong Kang, Meixian Wang, Yanqun Liu, Guicun Wang, Ping Yu, Juan Liang, Zhen Liu

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In one paragraph

Article in BMC pregnancy and childbirth, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Wenli Xu *National Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Lu Li *National Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Hong KangNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Meixian WangNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Yanqun LiuDepartment of Obstetrics and Gynecology, Renshou County Maternity and Child Hospital, Meishan, Sichuan, China.
Guicun WangDepartment of Obstetrics and Gynecology, Huize County Maternity and Child Hospital, Qujing, Yunnan, China.
Ping YuNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Juan LiangNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Zhen LiuNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. Jenny_liu@scu.edu.cn.ORCID http://orcid.org/0000-0003-0174-758X

Funding

the Applied Basic Research Program of Sichuan Province 2021YJ0212the National Natural Science Foundation of China 82103858the National Natural Science Foundation of Sichuan Province for Young Scholars 2023NSFSC1642the Popularization Application Project of Sichuan Province 21PJ057the Special Project for Basic Work of Science & Technology 2014FY110700
6 · The paper itself

Abstract

BACKGROUND AND

aimsRecent studies have indicated possible links between the microbiota and the fetal heart, while the relevant mechanism is still unknown. This study is aims to investigate whether analyzing the microbiota and metabolic profiles of amniotic fluid collected from pregnant women whose fetuses with or without non-syndromic congenital heart defects (CHDs), during the second and third trimester of pregnancy, could offer valuable insights into CHDs. METHODS AND

resultsA case-control study was conducted with 17 cases diagnosed with non-syndromic CHDs (CHDs group) and 34 controls without congenital anomalies (control group) at a ratio of 1:2. The 16 S rDNA gene sequencing and metabolomics methods were employed to assess 51 amniotic fluid samples. The amniotic fluid microbiome from the CHDs group exhibited significantly higher Shannon and Simpson indices compared to the control group. At the genus level, 240 bacterial taxa were substantially enriched in the two groups, with 93 of those taxa being highly enriched in the case group. Compared to the control group, the case group exhibited 177 metabolites that were significantly increased and 480 metabolites that were down-regulated. The differential metabolites were primarily enriched in the steroid hormone biosynthesis, bile secretion and ovarian steroidogenesis, according to KEGG analysis. The observed variations in nine metabolites could attributed to fifty-eight distinct bacterial taxa. The nine differential metabolites were mainly associated with pathways involving steroid hormone biosynthesis, bile secretion, glycolysis, tricarboxylic acid (TCA) cycle, NADPH metabolism, and acyl transfer pathways.

conclusionThe CHDs group has disturbed amniotic fluid microbiota and metabolites, and more research was required to elucidate the mechanism.

Indexed as

Amniotic FluidHeart Defects, CongenitalMicrobiotaAdultCase-Control StudiesFemaleHumansMetabolomeMetabolomicsMultiomicsPregnancyPregnancy Trimester, SecondPregnancy Trimester, ThirdBirth developmentCongenital heart defectsMetaboliteMicrobiotaMultiomics analysis

Identifiers

PMID39922995
PMCPMC11806881

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