Evidence map›Paper›PMID 39921736›Full record

ArticleMarine biotechnology (New York, N.Y.)2025

Characterization of Delta-7 Alkenone Desaturase in Haptophyte Gephyrocapsa huxleyi Through Heterologous Expression in Tisochrysis lutea.

Kohei Yoneda, Chinatsu Kobayashi, Hiroya Araie, Rikuri Morita, Ryuhei Harada, Yasuteru Shigeta, Hirotoshi Endo, Yoshiaki Maeda, Iwane Suzuki

Abstract read
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. ACS omega · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kohei Yoneda *Institute of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. yoneda.kohei.gt@u.tsukuba.ac.jp.ORCID http://orcid.org/0000-0003-0734-0617
Chinatsu Kobayashi *Graduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
Hiroya AraieDepartment of Biosciences, College of Science and Technology, Kanto Gakuin University, Mutsuura-Higashi, Kanazawa-Ku, Yokohama, Kanagawa, 236-8501, Japan.
Rikuri MoritaCenter for Computational Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Ryuhei HaradaCenter for Computational Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Yasuteru ShigetaCenter for Computational Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Hirotoshi EndoNational Institute of Technology, Tsuruoka College, 104 Sawada, Inooka, Tsuruoka, Yamagata, 997-8511, Japan.
Yoshiaki MaedaInstitute of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
Iwane SuzukiInstitute of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.

Funding

Japan Science and Technology Agency ARA02205Japan Society for the Promotion of Science 23K14001
6 · The paper itself

Abstract

The marine haptophyte Gephyrocapsa huxleyi is an ecologically and geochemically important phytoplankton due to its contribution to the global carbon cycle and its ability to biosynthesize certain alkenones. These alkenones are long-chain alkyl ketones with two to four trans-type double bonds. The genes encoding alkenone desaturase in G. huxleyi have not been experimentally characterized so far, partly due to the difficulty of inducing genetic transformation in G. huxleyi. Therefore, we introduced the putative alkenone delta-7 desaturase of G. huxleyi (designated "DesT") to the transformable and alkenone-producing haptophyte Tisochrysis lutea. We found two types of coding sequences for DesT, which are probably derived from the expression products of different alleles, and designated them "DesT-1" and "DesT-2." The ratio of C

Indexed as

HaptophytaKetonesAmino Acid SequenceCloning, MolecularKetonesAlkenonesDesaturaseGephyrocapsa huxleyiHeterologous expressionTisochrysis luteaTransformation

Identifiers

PMID39921736
PMCPMC11807052

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.