ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Structure-Guided Engineering of a Versatile Urethanase Improves Its Polyurethane Depolymerization Activity.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Directed Evolution of an Efficient Polycarbonate Depolymerase With Exceptional Operational Stability.Angewandte Chemie (International ed. in English) · 2026Article
- Polyurethane Cascade Depolymerization by a Combination of Thermal Pretreatment and Enzymatic Hydrolysis.ChemSusChem · 2026Article
- Closing the Loop on Polyurethane Foam Waste: Challenges, Emerging Technologies, and the Road to Sustainable Circularity.ACS sustainable chemistry & engineering · 2026Review
- A Modular Biosensor Platform for the Detection of Plastic Monomers and the Engineering of Promiscuous Amidases Toward Challenging Substrates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Insights into the Catalytic Activity of a Metagenome-Derived Urethanase.Journal of the American Chemical Society · 2025Article
- The discovery of new metagenomic urethanases utilising a novel colorimetric assay for applications in the biodegradation of polyurethanes.Green chemistry : an international journal and green chemistry resource : GC · 2025Article
- Identification of Urethanases for Biocatalytic Recycling of Toluene Diisocyanate- and Methylene Diphenyl Diisocyanate-Based Polyurethanes.ChemSusChem · 2025Article
- An Update: Enzymatic Synthesis for Industrial Applications.Angewandte Chemie (International ed. in English) · 2025Review
- Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay.ACS catalysis · 2025Article
- Standardization guidelines and future trends for PET hydrolase research.Nature communications · 2025Review
- The macromolecular crystallography beamlines of the Helmholtz-Zentrum Berlin at the BESSY II storage ring: history, current status and future directions.Journal of synchrotron radiation · 2025Article
- Structure-Guided Engineering of a Versatile Urethanase Improves Its Polyurethane Depolymerization Activity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Unveiling the enzymatic pathway of UMG-SP2 urethanase: insights into polyurethane degradation at the atomic level.Chemical science · 2025Article
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
Polyurethane (PUR), the fifth most prevalent synthetic polymer, substantially contributes to the global plastic waste problem. Biotechnology-based recycling methods have recently emerged as innovative solutions to plastic waste disposal and sparked interest among scientific communities and industrial stakeholders in discovering and designing highly active plastic-degrading enzymes. Here, the ligand-free crystal structure of UMG-SP2, a metagenome-derived urethanase with depolymerization activities, at 2.59 Å resolution, as well as its (co-)structures bound to a suicide hydrolase inhibitor and a short-chain carbamate substrate at 2.16 and 2.40 Å resolutions, respectively, is reported. Structural analysis and molecular dynamics simulations reveal that the flexible loop L3 consisting of residues 219-226 is crucial for regulating the hydrolytic activity of UMG-SP2. The semi-rational redesign of UMG-SP2 reveals superior variants, A141G and Q399A, exhibiting over 30.7- and 7.4-fold increased activities on polyester-PUR and a methylene diamine derivative of PUR, respectively, compared to the wild-type enzyme. These findings advance the understanding of the structure-function relationship of PUR-hydrolyzing enzymes, which hold great promise for developing effective industrial PUR recycling processes and mitigating the environmental footprint of plastic waste.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.