Evidence map›Paper›PMID 39920625›Full record

ArticleBMC genomics2025

Autophagy is involved in the toxicity of the biocontrol agent GC16 against Tetranychus pueraricola (Acari: Tetranychidae) based on transcriptomic and proteomic analyses.

Yanyan He, Guangzu Du, Guang Wang, Huiming Guan, Shusheng Zhu, Bin Chen, Xiahong He, Youyong Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yanyan HeSchool of Agriculture, Yunnan University, Kunming, 650500, China.
Guangzu DuState Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
Guang WangState Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
Huiming GuanInner Mongolia Wulanchabu Science and Technology Development Center, Wulanchabu, 012000, China.
Shusheng ZhuState Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
Bin ChenState Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China. chbins@163.com.
Xiahong HeSouthwest Forestry University, Kunming, 650224, China. hexiahong@hotmail.com.
Youyong ZhuSchool of Agriculture, Yunnan University, Kunming, 650500, China. yyzhu@ynau.edu.cn.

Funding

the Major Science and Technology Project of the Yunnan Provincial Science and Technology Department NO.202102AE090042-02-06the Yunnan provincial Municipal Education Commission Innovation Team Project 2022[69]
6 · The paper itself

Abstract

backgroundGC16 is a novel pesticide with acaricidal properties against the spider mite Tetranychus pueraricola (Ehara & Gotoh). Its physiological mechanisms have been described previously, but its molecular mechanisms of action remain unclear. Thus, we aimed to explore the acaricidal mechanisms of GC16 through transcriptomic and proteomic analyses. The results were verified using transmission electron microscopy (TEM), immunofluorescence assay, and western blotting.

resultsTranscriptomic and proteomic analyses revealed 2717 differentially expressed genes (DEGs) and 374 differentially expressed proteins (DEPs) between the GC16-treated and control mites. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that the DEGs and DEPs were enriched in the autophagy pathway. TEM showed that the number of autophagosomes and autolysosomes was higher in the GC16-treated mites than in the control mites. Immunofluorescence assay and western blot results consistently indicated that GC16 treatment significantly enhanced the relative expression of the autophagy protein LC3 in insect Sf9 cells. The intracellular calcium concentration in the GC16-treated Sf9 cells was 2.30 times higher than that in the control cells, suggesting that GC16 disrupted calcium homeostasis and potentially acted as a calcium-driven nerve agent.

conclusionsAutophagy is involved in the toxicity of GC16 against T. pueraricola and may be activated by elevated Ca

Indexed as

AcaricidesAutophagyBiological Control AgentsTetranychidaeTranscriptomeAnimalsCalciumGene Expression ProfilingProteomeProteomicsSf9 CellsAcaricidesBiological Control AgentsCalciumProteomeAutophagyGC16 biopesticideProteomicTetranychus PueraricolaToxicityTranscriptomic

Identifiers

PMID39920625
PMCPMC11806590

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.