Evidence map›Paper›PMID 39920532›Full record

ArticleThe protein journal2025

From Crude Extracts to Purity: A Comparative Study of Butyrylcholinesterase Purification.

Gamze Sonmez, Bahattin Enes Karatas, Ebru Bodur

Erratum issuedAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in The protein journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Gamze SonmezDepartment of Medical Biochemistry, Hacettepe University Faculty of Medicine, Ankara, Turkey.ORCID http://orcid.org/0009-0001-3488-0283
Bahattin Enes KaratasDepartment of Medical Biochemistry, Hacettepe University Faculty of Medicine, Ankara, Turkey.ORCID http://orcid.org/0009-0008-6070-0769
Ebru BodurDepartment of Medical Biochemistry, Hacettepe University Faculty of Medicine, Ankara, Turkey. ebodur@hacettepe.edu.tr.ORCID http://orcid.org/0000-0001-5829-5487

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Butyrylcholinesterase (BChE; EC 3.1.1.8) is an enzyme found in blood plasma and various tissues, playing a key role in metabolizing esters and detoxifying various substances. In this study, we developed a modified purification protocol for BChE from human serum, achieving a higher purification yield (38.3%) and specific activity (15.09 U/mg protein) compared to previous reports. The method employed a single round of acid dialysis, Sephadex G50 gel filtration chromatography, and procainamide Sepharose 4 fast flow affinity chromatography. Our new approach excludes the commonly used DEAE Trisacryl M chromatography. The goal was to compare this method with our previously employed purification protocols. This study demonstrates that optimizing chromatography steps can enhance enzyme recovery and activity, though further refinement may be needed for higher purification folds. This improved methodology offers a valuable approach for efficient BChE purification with potential for broader applications.

Indexed as

ButyrylcholinesteraseChromatography, AffinityChromatography, GelHumansButyrylcholinesteraseAffinity chromatographyButyrylcholinesteraseGel filtration chromatographyPolyacrylamide gel electrophoresisPurification

Identifiers

PMID39920532

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.