Evidence map›Paper›PMID 39918763›Full record

ArticleJournal of assisted reproduction and genetics2025

Spermatozoa centriole quality determined by FRAC may correlate with zygote nucleoli polarization-a pilot study.

Derek F Kluczynski, Ankit Jaiswal, Min Xu, Nagalakshmi Nadiminty, Barbara Saltzman, Samantha Schon, Tomer Avidor-Reiss

Abstract read
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In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Derek F KluczynskiDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, USA.ORCID http://orcid.org/0000-0003-0977-4961
Ankit JaiswalDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, USA.ORCID http://orcid.org/0000-0002-0078-3602
Min XuDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0009-0009-1335-0580
Nagalakshmi NadimintyDepartment of Urology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, USA.ORCID http://orcid.org/0000-0003-3408-3206
Barbara SaltzmanDepartment of Population Health, College of Health and Human Services, University of Toledo, Toledo, OH, USA.ORCID http://orcid.org/0000-0002-7337-5749
Samantha SchonDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0001-5524-3649
Tomer Avidor-ReissDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, USA. tomer.avidorreiss@utoledo.edu.ORCID http://orcid.org/0000-0003-0918-526X

Funding

The Role of Rabbit POC1B inSperm CentriolesR15HD110863 · NICHD · UNIVERSITY OF TOLEDO · PI AVIDOR-REISS, TOMER · 2023 to 2023
$452k
National Institute of Child Health and Human Development R15HD110863NICHD NIH HHS R15 HD110863
6 · The paper itself

Abstract

purposeSpermatozoa centriolar defects can result in abnormal zygote functions. Recently, a method to quantify spermatozoa centriolar defects was developed named Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC). However, whether spermatozoa centriolar defects identified by FRAC can result in abnormal zygote functions was not tested.

methodsHere, we quantified spermatozoa centriolar defects using FRAC, and zygote centriole function was assessed by imaging Nucleolus Precursor Body (NPB) polarization which was based on the pattern of NPB polarization. Data was analyzed at couple and embryo levels. Subjects were divided into two groups: seven couples and 62 embryos with normal spermatozoa centrioles versus eight couples and 78 embryos with abnormal spermatozoa centrioles (140 embryos from 15 couples in total).

resultsPatterned NPB polarization was statistically significant in both couple- and embryo-level analyses (p < 0.0001 and p = 0.0024). These results suggest that the abnormal spermatozoa centrioles identified by FRAC may correlate with abnormal zygote centrosome function via NPB polarization scoring.

conclusionsThis study provides a foundation for more extensive studies to test for FRAC's utility in assessing spermatozoa centriole quality.

Indexed as

Cell NucleolusCentriolesSpermatozoaZygoteAdultCentrosomeFemaleHumansMalePilot ProjectsCentrioleInfertilityNucleolus Precursor BodyReproductionSpermatozoaZygote

Identifiers

PMID39918763
PMCPMC12055725

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.