Evidence map›Paper›PMID 39918761›Full record

ArticlePlant molecular biology2025

The role of the sucrose synthase gene in promoting thorn occurrence and vegetative growth in Lycium ruthenicum.

Wenhui Liu, Weiman Xu, Yue Gao, Xinyu Qi, Fuqiang Liu, Jiawen Wang, Lujia Li, Yuliang Zhou, Wenxin Chen, Yingyue Jiang and 3 more

Abstract read
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In one paragraph

Article in Plant molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wenhui LiuKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Weiman XuKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Yue GaoKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Xinyu QiKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Fuqiang LiuKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Jiawen WangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Lujia LiKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Yuliang ZhouKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Wenxin ChenKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Yingyue JiangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Jianguo CuiKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Yucheng WangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Qin-Mei WangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China. wqmwan@syau.edu.cn.ORCID http://orcid.org/0000-0003-0996-8459

Funding

National Natural Science Foundation of China 32171831Natural Science Foundation of Liaoning Province 2023-MS-207Scientific Research Fund of Liaoning Provincial Education Department JYTYB2024040
6 · The paper itself

Abstract

Lycium ruthenicum is a highly valued ecological and economic shrub, but its abundant thorns disrupt production processes. Previous studies suggested that the sucrose synthase gene (LrSUS) in L. ruthenicum may influence thorn occurrence, presenting potential for breeding thornless varieties suited for cultivation. To explore this, the full-length CDS of LrSUS was cloned, and a novel stable genetic transformation system mediated by Agrobacterium tumefaciens was developed. Through this system, both LrSUS overexpression and suppression lines were generated. While suppression lines exhibited slow growth and failed to survive post-transplant, overexpression lines demonstrated accelerated growth, with significant increases in adventitious root number and length. Upon transplanting, the overexpression lines also showed enhanced thorn occurrence, alongside notable increases in thorn length, leaf size, stem diameter, photosynthetic rate, and sugar content. Subcellular localization analysis using a transient expression method based on the injection of L. ruthenicum indicated that the LrSUS gene product is localized in the chloroplasts. Key genes involved in LrSUS/ sucrose affecting thorn occurrence event were identified through high throughput transcriptome analysis and a hypothetical mechanistic model was established. This study provides valuable insights into the function of LrSUS and establishes a foundation for manipulating thorn phenotypes in L. ruthenicum and related species.

Indexed as

GlucosyltransferasesLyciumPlant ProteinsChloroplastsGene Expression Regulation, PlantPlant LeavesPlants, Genetically ModifiedSucroseGlucosyltransferasesPlant ProteinsSucrosesucrose synthaseLycium ruthenicumRNAiSubcellular localizationThornTransgene

Identifiers

PMID39918761

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.