Evidence map›Paper›PMID 39918276›Full record

ArticleAnimal biotechnology2025

Study on immortalization of Mongolian sheep fibroblast cells.

Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Polymorphisms in the 3'UTR of theFrontiers in veterinary science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Bin LiuCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, People's Republic of China.
Shichao WangDepartment of Medical Laboratory, Hohhot First Hospital, Hohhot, Inner Mongolia, People's Republic of China.
Fanhua MengCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, People's Republic of China.
Bei WuCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, People's Republic of China.
Yanru ZhangCollege of Medicine, Hainan Vocational University of Science and Technology, Haikou, Hainan, People's Republic of China.
Junwei CaoCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, People's Republic of China.ORCID 0009-0005-8205-4364

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to establish an immortalized fibroblast cell line from Mongolian sheep. Primary Mongolian sheep fibroblasts (SSF) were isolated using tissue explant and enzymatic digestion methods, followed by microscopic observation, growth curve plotting, and karyotype analysis. The results confirmed the successful isolation of SSF. Human (hTERT) and sheep (sTERT) telomerase reverse transcriptase vectors were separately introduced into SSF, with cells passaged up to 36 generations following G418 selection. Microscopic examination and qRT-PCR results demonstrated that TERT transfection did not alter the morphology of SSF and led to stable, high levels of TERT expression (

Indexed as

FibroblastsAnimalsCell LineCell ProliferationCell SurvivalCellular SenescenceHumansSheepTelomeraseTransfectionTelomeraseTERT protein, humanfibroblasthuman telomerase reverse transcriptaseimmortalizationMongolian sheepsheep telomerase reverse transcriptase

Identifiers

PMID39918276
PMCPMC12674303

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.