Evidence map›Paper›PMID 39917634›Full record

ArticleFrontiers in cellular and infection microbiology2025

Visible and rapid detection of feline chaphamaparvovirus using multienzyme isothermal rapid amplification and lateral flow dipstick assay.

Jun Ji, Xinhao Mu, Shunshun Pan, Xin Xu, Shiyuan Zhang, Honghui Huang, Ying Li, Yingzuo Bi, Lunguang Yao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jun JiHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Xinhao MuHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Shunshun PanHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Xin XuHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Shiyuan ZhangHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Honghui HuangHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Ying LiHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.
Yingzuo BiCollege of Animal Science and Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Lunguang YaoHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feline chaphamaparvovirus (FeChPV) is a novel parvovirus previously reported in Canadian cats and Chinese dogs with diarrhea in 2019 and 2020, respectively. Herein, we aimed to establish a simple detection method for FeChPV in field clinics. The primers and probes for the multienzyme isothermal rapid amplification and lateral flow dipstick (MIRA-LFD) assay were designed to target the conserved regions of the FeChPV genome and determine the optimal reaction temperature and time. Without relying on precision instruments, FeChPV detection using the MIRA-LFD assay was completed within 20 min at 37°C, without any cross-reaction with other reference viruses. The newly established MIRA-LFD assay had a detection limit of 32.3 copies/μL, which was 10-fold lower than that of the nested polymerase chain reaction (PCR) assay. Furthermore, the MIRA-LFD assay detected 29 FeChPV-positive samples among 417 cats with diarrhea, providing a slightly higher positivity rate than the nested PCR assay. These results indicate that the newly developed MIRA-LFD assay for FeChPV detection is an efficient, economical, reliable, and simple method that can help in the early prevention and control of FeChPV infection.

Indexed as

Cat DiseasesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesParvoviridae InfectionsAnimalsCatsDiarrheaDNA PrimersFecesSensitivity and SpecificityTemperatureDNA PrimersdetectionFeChPVMIRA-LFDsensitivitysimplicity

Identifiers

PMID39917634
PMCPMC11794484

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.