Evidence map›Paper›PMID 39917185›Full record

ArticleJournal of applied crystallography2025

Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography.

Clare De'Ath, Mizar F Oliva, Martine Moulin, Matthew P Blakeley, Michael Haertlein, Edward P Mitchell, José A Gavira, Matthew W Bowler, V Trevor Forsyth

Abstract read
In one paragraph

Article in Journal of applied crystallography, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Clare De'AthLife Sciences Group Institut Laue-Langevin 71 Avenue des Martyrs Grenoble38042 France.
Mizar F OlivaLife Sciences Group Institut Laue-Langevin 71 Avenue des Martyrs Grenoble38042 France.ORCID https://orcid.org/0000-0002-1204-1761
Martine MoulinLife Sciences Group Institut Laue-Langevin 71 Avenue des Martyrs Grenoble38042 France.ORCID https://orcid.org/0000-0002-2212-4368
Matthew P BlakeleyLarge Scale Structures Group Institut Laue-Langevin 71 Avenue des Martyrs Grenoble38042 France.ORCID https://orcid.org/0000-0002-6412-4358
Michael HaertleinLife Sciences Group Institut Laue-Langevin 71 Avenue des Martyrs Grenoble38042 France.
Edward P MitchellEuropean Synchrotron Radiation Facility 71 Avenue des Martyrs Grenoble38042 France.
José A GaviraLaboratorio de Estudios Cristalográficos Instituto Andaluz de Ciencias de la Tierra (CSIC) Av. Las Palmeras 4 Granada18100 Spain.ORCID https://orcid.org/0000-0002-7386-6484
Matthew W BowlerEuropean Molecular Biology Laboratory, 71 Avenue des Martyrs, Grenoble38042, France.ORCID https://orcid.org/0000-0003-0465-3351
V Trevor ForsythMedical Faculty Lund University SE-221 84Lund Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.

Indexed as

amyloidogenesiscounter-diffusion crystallizationcrystallogenesislarge-volume crystalsmacromolecular deuterationneutron macromolecular crystallographyperdeuterationtransthyretinX-ray diffraction

Identifiers

PMID39917185
PMCPMC11798515

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.