Evidence map›Paper›PMID 39917018›Full record

ArticleFrontiers in bioengineering and biotechnology2025

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation.

Elin Arvidsson, Diana Duarte Lobo, Ermelinda Sabarese, Fabio Duarte, Rui Jorge Nobre, Luis Quintino, Cecilia Lundberg

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Elin ArvidssonCNS Gene Therapy, Department of Experimental Medical Sciences, Lund University, Lund, Sweden.
Diana Duarte LoboCNC - Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra, Coimbra, Portugal.
Ermelinda SabareseCNS Gene Therapy, Department of Experimental Medical Sciences, Lund University, Lund, Sweden.
Fabio DuarteCNS Gene Therapy, Department of Experimental Medical Sciences, Lund University, Lund, Sweden.
Rui Jorge NobreCNC - Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra, Coimbra, Portugal.
Luis QuintinoCNS Gene Therapy, Department of Experimental Medical Sciences, Lund University, Lund, Sweden.
Cecilia LundbergCNS Gene Therapy, Department of Experimental Medical Sciences, Lund University, Lund, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR-mediated gene activation (CRISPRa) encompasses a growing field of biotechnological approaches with exciting implications for gene therapy. However, there is a lack of experimental validation tools for selecting efficient sgRNAs for downstream applications. Here, we present a screening assay capable of identifying efficient single- and double sgRNAs through fluorescence quantification

Indexed as

Adam17CRISPRagene activationgene therapySirt1Tfeb

Identifiers

PMID39917018
PMCPMC11799263

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.