Evidence map›Paper›PMID 39915769›Full record

ArticleBMC pulmonary medicine2025

A case report of confirmed difficult pulmonary tuberculosis based on the hybrid capture-based tNGS method.

Weiqian Chen, Huimin Chen, Ze Liu, Xinle Chi, Yaomeng Chen, Huan Ye, Wenjie Huang, Chenlei Cao, Wei Weng

Abstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pulmonary medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Weiqian Chen *Department of Respiratory Medicine, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, 325000, Zhejiang, China.ORCID http://orcid.org/0000-0003-2937-1545
Huimin Chen *Department of Cardiology, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, 325000, Zhejiang, China.
Ze LiuDepartment of Nuclear Medicine, Ningbo Hangzhou Bay Hospital, Ningbo, 315327, Zhejiang, China.ORCID http://orcid.org/0009-0004-8367-0970
Xinle ChiDepartment of Radiology, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, 325000, Zhejiang, China.ORCID http://orcid.org/0009-0009-7833-0935
Yaomeng ChenDepartment of Radiology, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, 325000, Zhejiang, China.ORCID http://orcid.org/0009-0007-4833-5044
Huan YeDepartment of Respiratory Medicine, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, 325000, Zhejiang, China.
Wenjie HuangDepartment of Nuclear Medicine, Xinqiao Hospital, Chongqing, 400037, China.
Chenlei CaoDepartment of Respiratory Medicine, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, 325000, Zhejiang, China.
Wei WengDepartment of Radiology, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, 325000, Zhejiang, China. wengwei318@163.com.ORCID http://orcid.org/0009-0008-0920-1446

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEarly diagnosis of pulmonary tuberculosis can greatly reduce the harm caused by the disease. However, traditional diagnostic methods have various shortcomings in diagnosing pulmonary tuberculosis. Currently, with the increasing popularity, iteration, and decreasing costs of Next-generation sequencing (NGS) testing technology, NGS is being more widely applied in the diagnosis of pulmonary tuberculosis. CASE PRESENTATION: A 29-year-old male presented with "fever accompanied by cough for more than 20 days." Multiple chest CT scans revealed progressive enlargement of the right hilar lymph nodes and thickening of the interlobular septa in the right upper lobe. Routine testing of bronchoalveolar lavage fluid, search for tuberculosis bacilli, bacterial and fungal cultures, X-pert MTB/RIF, and multiplex PCR-based targeted Next-generation sequencing (mp-tNGS) results were all inconclusive. Finally, bronchoalveolar lavage fluid was sent for hybrid capture-based targeted Next-generation sequencing (hc-tNGS) testing, and special staining of the enlarged lymph nodes confirmed the diagnosis of pulmonary tuberculosis.

conclusionThe hc-tNGS has significant value in diagnosing pulmonary tuberculosis, especially in cases that are difficult to detect with other methods. In the future, this could gradually become a routine diagnostic method for pulmonary tuberculosis, enhancing the accuracy of early diagnosis.

Indexed as

High-Throughput Nucleotide SequencingMycobacterium tuberculosisTuberculosis, PulmonaryAdultBronchoalveolar Lavage FluidHumansLymph NodesMaleTomography, X-Ray ComputedCase reportDiagnostic methodsHybrid capture-based tNGSPulmonary tuberculosis

Identifiers

PMID39915769
PMCPMC11800540

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