Evidence map›Paper›PMID 39914390›Full record

ArticleDevelopmental cell2025

Liquid-like condensates that bind actin promote assembly and bundling of actin filaments.

Caleb Walker, Aravind Chandrasekaran, Daniel Mansour, Kristin Graham, Andrea Torres, Liping Wang, Eileen M Lafer, Padmini Rangamani, Jeanne C Stachowiak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Caleb WalkerBiomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Aravind ChandrasekaranDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Daniel MansourDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Kristin GrahamCell and Molecular Biology, The University of Texas at Austin, Austin, TX, USA.
Andrea TorresBiomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Liping WangDepartment of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Eileen M LaferDepartment of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Padmini RangamaniDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA; Department of Pharmacology, University of California, San Diego School of Medicine, La Jolla, CA, USA. Electronic address: prangamani@ucsd.edu.
Jeanne C StachowiakBiomedical Engineering, The University of Texas at Austin, Austin, TX, USA; Chemical Engineering, The University of Texas at Austin, Austin, TX, USA. Electronic address: jcstach@austin.utexas.edu.

Funding

Protein Networks as Synergistic Drivers of Membrane RemodelingR35GM139531 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Jeanne Casstevens Stachowiak · 2021 to 2026
$4.0M
Modeling and analysis of the mechanochemical processes that govern clathrin-mediated endocytosisR01GM132106 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RANGAMANI, PADMINI · 2020 to 2024
$1.5M
NIGMS NIH HHS R01 GM132106NIGMS NIH HHS R35 GM139531
6 · The paper itself

Abstract

Biomolecular condensates perform diverse physiological functions. Previous work showed that VASP, a processive actin polymerase, forms condensates that assemble and bundle actin. Here, we show that this behavior does not require proteins with specific polymerase activity. Specifically, condensates composed of Lamellipodin, a protein that binds actin but is not an actin polymerase, were also capable of assembling actin filaments. To probe the minimum requirements for condensate-mediated actin bundling, we developed an agent-based computational model. Guided by its predictions, we hypothesized that any condensate-forming protein that binds filamentous actin could bundle filaments through multivalent crosslinking. To test this, we added a filamentous-actin-binding motif to Eps15, a condensate-forming protein that does not normally bind actin. The resulting chimera formed condensates that facilitated efficient assembly and bundling of actin filaments. Collectively, these findings broaden the family of proteins that could organize cytoskeletal filaments to include any filamentous-actin-binding protein that participates in protein condensation.

Indexed as

Actin CytoskeletonActinsBiomolecular CondensatesMicrofilament ProteinsAnimalsHumansProtein BindingActinsMicrofilament Proteinsactin cytoskeletonagent-based modelingbiomolecular condensatesLamellipodinmultivalent interactionsprotein phase separationVASP

Identifiers

PMID39914390
PMCPMC12151779

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.