ArticleBiotechnology and bioengineering2025
Systematic Development of a Detergent Toolbox as an Alternative to Triton X-100.
Article in Biotechnology and bioengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Design Space Assessment of Virus Inactivation in Plasma-Derived Matrices Establishes a Single Detergent Alternative to TX-100-Containing Mixes.Biotechnology and bioengineering · 2026Article
- Downstream Purification Strategies for Virus-like Particles: A Systematic Review of Structure Preservation, Impurity Control, and Viral Safety.Microorganisms · 2026Review
- Detergent-Triggered Membrane Remodelling Monitored via Intramembrane Fluorescence Dequenching.ACS omega · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detergents are routinely included in protein purification processes to inactivate enveloped viruses that may arise from adventitious or endogenous contamination. The detergent Triton X-100 (TX-100) has been widely used as part of the production process for therapeutic proteins. However, recent ecological studies indicate that TX-100 and its metabolites detrimentally impact aquatic organisms, thus alternative detergents for viral inactivation are required. The overall aim of this study was to identify one or more detergents that are a suitable replacement for TX-100 in the viral inactivation step. In stage one, 16 potential alternatives were identified and screened against TX-100 using multiple criteria such as solubility, feasibility of virus inactivation, critical micelle concentration, and storage conditions. The multi-criteria decision analysis (MCDA) methodology was used to identify four candidates for the second stage assessment. In stage two, a detailed evaluation was undertaken and two candidates C16-AO, and C11/15-sEO9, were found to be practical alternatives to TX-100 for use in protein therapeutic production processes for inactivating enveloped viruses. In addition, C13-EO8 demonstrated good viral inactivation capability and warrants further investigation in detergent clearance and impact on product quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.