Evidence map›Paper›PMID 39910557›Full record

ArticlePlant methods2025

In vitro micropropagation protocols for two endangered Dianthus species - via in vitro culture for conservation and recultivation purposes.

Dóra Farkas, Judit Csabai, Angéla Kolesnyk, Pál Szarvas, Judit Dobránszki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Plant biotechnology (Tokyo, Japan) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dóra FarkasCentre for Agricultural Genomics and Biotechnology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, Nyíregyháza, H-4400, Hungary.ORCID http://orcid.org/0009-0006-6101-6104
Judit CsabaiInstitute of Engineering and Agricultural Sciences, University of Nyíregyháza, Sóstói str. 31/b, Nyíregyháza, H-4400, Hungary.ORCID http://orcid.org/0000-0002-5550-3399
Angéla KolesnykDepartment of Genetics, Plant Biology and Microbiology, Uzhhorod National University, 3 Narodna Square, Uzhhorod, 88000, Ukraine.ORCID http://orcid.org/0000-0001-8781-3335
Pál SzarvasCentre for Agricultural Genomics and Biotechnology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, Nyíregyháza, H-4400, Hungary.ORCID http://orcid.org/0009-0007-3784-4038
Judit DobránszkiCentre for Agricultural Genomics and Biotechnology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, Nyíregyháza, H-4400, Hungary. dobranszki@freemail.hu.ORCID http://orcid.org/0000-0001-7624-6398

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundD. giganteiformis subsp. pontederae and D. superbus subsp. superbus are protected or critically endangered species in several European regions; therefore, developing an efficient in vitro micropropagation protocol is essential for germplasm conservation and recultivation purposes.

resultsAfter germination, one-nodal segments of both species were transferred onto several MS media supplemented with 3% sucrose and different types of cytokinins (at a concentration of 4.5 µM) alongside 0.54 µM 1-naphthaleneacetic acid (NAA) for the multiplication phase for 3 weeks. The shoot clusters were subsequently transferred onto elongation medium (plant growth regulator-free MS medium) for 3 weeks. Individual shoots separated from the shoot clusters were cultured on MS medium supplemented with 0.54 µM NAA and 2% sucrose for 3 weeks for rooting. Taking into account the effects and after-effects of cytokinins, we found that the most suitable cytokinin for D. giganteiformis subsp. pontederae was N-(2-isopentenyl)-adenine (2-iP), while for D. superbus subsp. superbus it was meta-topolin (mT).

conclusionsIn vitro micropropagation methods were developed for two endangered Dianthus species (D. giganteiformis subsp. pontederae and D. superbus subsp. superbus) by determining the optimal type of cytokinin to be used during the multiplication phase. The protocols are designed to produce large quantities of propagation material for recultivation, educational, and research purposes within three months.

Indexed as

CytokininsDisordersGenotype dependenceMicropropagationOptimal growth index

Identifiers

PMID39910557
PMCPMC11796268

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.