Evidence map›Paper›PMID 39907710›Full record

ArticleBiotechnology letters2025

Comparative study of nanohydroxyapatite-emdogain effects on apical papilla stem cell survival and differentiation.

Elham Khoshbin, Hamed Karkehabadi, Razieh Salehi, Abbas Farmany, Rezvan Najafi, Roshanak Abbasi

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Elham KhoshbinDepartment of Endodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.ORCID http://orcid.org/0000-0002-5068-8080
Hamed KarkehabadiDepartment of Endodontics, Dental Research Center, Avicenna Institute of Clinical Sciences, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.ORCID http://orcid.org/0000-0002-1296-7361
Razieh SalehiDepartment of Endodontics, School of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran.ORCID http://orcid.org/0000-0002-7783-2871
Abbas FarmanyDental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.ORCID http://orcid.org/0000-0003-1752-804X
Rezvan NajafiDepartment of Medical Molecular & Genetics, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.ORCID http://orcid.org/0000-0003-1326-1288
Roshanak AbbasiDepartment of Endodontics, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran. abbasi.roshanak@gmail.com.ORCID http://orcid.org/0000-0001-5109-4674

Funding

Vice Chancellor for Research and Technology, Hamadan University of Medical Sciences 9811158833
6 · The paper itself

Abstract

backgroundThe study was designed to explore the enhanced impact of nano-hydroxyapatite and emdogain on the survival and osteogenic/odontogenic differentiation of human stem cells isolated from the apical papilla (hSCAPs). MATERIALS AND

methodsIn this in vitro trial, hSCAPS obtained from intact impacted immature third molars were confirmed to have characteristic cell surface markers, then exposed to nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain for durations of 1-3 days. The survival of apical papilla stem cells was measured using a methyl thiazolyl tetrazolium assay. The quantitative reverse transcription polymerase chain reaction, alkaline phosphatase activity (ALP) and Alizarin red staining were used to evaluate osteogenic-odontogenic differentiation. Analysis of the data was done using one-way ANOVA, t-test, and Mann-Whitney test (α = 0.05).

resultsAt 1-3 days, emdogain exhibited no significant impact on the survival of human stem cells from the apical papilla. In contrast, nanohydroxyapatite (α > 0.05) and nanohydroxyapatite coated with emdogain demonstrated a notable reduction in cell survival compared to the control group (α < 0.05). The expression of dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein genes demonstrated a notable increase in the group treated with nanohydroxyapatite coated with emdogain compared to the other groups (α < 0.05), and furthermore, this group exhibited more pronounced mineralized nodules than the other experimental groups.

conclusionIn contrast to nanohydroxyapatite, Emdogain did not demonstrate a detrimental effect on the survival of hSCAPs. Nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain increased osteogenic/odontogenic differentiation of hSCAPs.

Indexed as

Cell DifferentiationDental PapillaDurapatiteStem CellsCells, CulturedCell SurvivalHumansNanoparticlesOsteogenesisDurapatiteCell differentiationCell survivalEmdogainHydroxyapatiteMesenchymal Stem cellsNanoparticles

Identifiers

PMID39907710

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.