Evidence map›Paper›PMID 39906960›Full record

ArticleCurrent protocols2025

Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells.

Amber Crabtree, Han Le, Chanel Harris, Ashton Oliver, Andy Barillas, Johnathan Moore, Desiree Ngoc-Ha Nguyen, Izabella Marie Rabago, Benjamin Rodriguez, Dominique C Stephens and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. The mitochondrial lactate oxidation complex: endpoint for carbohydrate carbon disposal.American journal of physiology. Endocrinology and metabolism · 2025
    Article
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

26 authors.

Amber CrabtreeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Han LeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Chanel HarrisDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Ashton OliverDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Andy BarillasDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Johnathan MooreDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Desiree Ngoc-Ha NguyenDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Izabella Marie RabagoDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Benjamin RodriguezDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Dominique C StephensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Edgar Garza-LopezDepartment of Internal Medicine, University of Iowa, Iowa City, Iowa.
Kit NeikirkDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Margaret MungaiDepartment of Internal Medicine, University of Iowa, Iowa City, Iowa.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Neng VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Kyrin TurnerDepartment of Life and Physical Sciences, Fisk University, Nashville, Tennessee.
Jianqiang ShaoCentral Microscopy Research Facility, University of Iowa, Iowa City, Iowa.
Sandra MurrayDepartment of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
Jennifer A GaddyDivision of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee.
Celestine WanjallaVanderbilt University Medical Center, Department of Medicine, Division of Infectious Disease, Nashville, Tennessee.
Jamaine DavisDepartment of Biochemistry and Cancer Biology, Meharry Medical College, Nashville, Tennessee.
Steven DamoDepartment of Life and Physical Sciences, Fisk University, Nashville, Tennessee.
Antentor O HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.ORCID https://orcid.org/0000-0002-7730-952X

Funding

VANDERBILT UNIVERSITY CTSA FOR PEDIATRIC RESEARCHUL1RR024975 · NCRR · VANDERBILT UNIVERSITY · PI BERNARD, GORDON RAPHAEL · 2007 to 2011
$45.7M
The Vanderbilt Institute for Clinical and Translational Research (VICTR)UL1TR000445 · NCATS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BERNARD, GORDON RAPHAEL · 2012 to 2016
$41.4M
Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ALVIN C POWERS · 2012 to 2026
$29.3M
CLINICAL PHARMACOLOGY TRAINING PROGRAMT32GM007569 · NIGMS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Bjorn C Knollmann · 1985 to 2026
$12.4M
PRIDE: Functional and Translational Genomics of Blood DisordersR25HL106365 · NHLBI · AUGUSTA UNIVERSITY · PI PACE, BETTY SUE · 2010 to 2024
$5.2M
Integrated Training in Engineering and DiabetesT32DK101003 · NIDDK · VANDERBILT UNIVERSITY · PI Jamey D. Young · 2014 to 2026
$3.9M
Determining the contribution of zinc deficiency to perinatal Group B Streptococcus infectionsR01HD090061 · NICHD · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ARONOFF, DAVID M, GADDY, JENNIFER A · 2017 to 2021
$2.5M
The Meharry Research Training InitiativeT32GM144927 · NIGMS · MEHARRY MEDICAL COLLEGE · PI Pamela M Martin · 2022 to 2026
$1.9M
BLRD VA I01 BX005352NCATS NIH HHS UL1 TR000445NCRR NIH HHS UL1 RR024975NHLBI NIH HHS R25 HL106365NICHD NIH HHS R01 HD090061NIDDK NIH HHS P30 DK020593NIDDK NIH HHS T32 DK101003NIGMS NIH HHS T32 GM007569NIGMS NIH HHS T32 GM144927
6 · The paper itself

Abstract

Proximity ligation assays (PLAs) use specific antibodies to detect endogenous protein-protein interactions. PLAs are a highly useful biochemical technique that allow two proteins within proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominence, the use of a PLA in mouse skeletal muscle (SkM) is novel. In this article, we discuss how the PLA method can be used in SkM to study the protein-protein interactions within mitochondria-endoplasmic reticulum contact sites (MERCs). © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Proximity ligation assay for skeletal muscle tissue and myoblast for MERC proteins.

Indexed as

Endoplasmic ReticulumMitochondriaMuscle, SkeletalAnimalsBiomarkersMiceBiomarkersMERCsMfn1Mfn2mitochondriaprotein‐protein interactionsproximity ligation assaysskeletal muscles

Identifiers

PMID39906960
PMCPMC11795341

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.