Evidence map›Paper›PMID 39906927›Full record

ArticleCurrent protocols2025

Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue.

Margaret Mungai, Amber Crabtree, Han Le, Johnathan Moore, Desiree Nguyen, Benjamin Rodriguez, Chanel Harris, Dominique C Stephens, Heather K Beasley, Edgar Garza-Lopez and 19 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

29 authors.

Margaret MungaiDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Amber CrabtreeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Han LeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Johnathan MooreDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Desiree NguyenDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Benjamin RodriguezDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Chanel HarrisDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Dominique C StephensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Edgar Garza-LopezDepartment of Internal Medicine, University of Iowa, Iowa City, Iowa.
Kit NeikirkDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Bryanna ShaoDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Ashton OliverDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Genesis WilsonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Serif BacevacCentral Microscopy Research Facility, University of Iowa, Iowa City, Iowa.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Neng VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Kyrin TurnerDepartment of Life and Physical Sciences, Fisk University, Nashville, Tennessee.
Elma ZaganjorDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Jianqiang ShaoCentral Microscopy Research Facility, University of Iowa, Iowa City, Iowa.
Sandra MurrayDepartment of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
Jennifer A GaddyDivision of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee.
Celestine WanjallaDivision of Infectious Disease, Vanderbilt University Medical Center: Department of Medicine, Nashville, Tennessee.
Jamaine DavisDepartment of Biochemistry and Cancer Biology, Meharry Medical College, Nashville, Tennessee.
Steven M DamoDepartment of Life and Physical Sciences, Fisk University, Nashville, Tennessee.
Lori D BanksDepartment of Biology, Prairie View A&M University, Prairie, View, Texas.
Antentor HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.ORCID https://orcid.org/0000-0002-7730-952X

Funding

VANDERBILT UNIVERSITY CTSA FOR PEDIATRIC RESEARCHUL1RR024975 · NCRR · VANDERBILT UNIVERSITY · PI BERNARD, GORDON RAPHAEL · 2007 to 2011
$45.7M
The Vanderbilt Institute for Clinical and Translational Research (VICTR)UL1TR000445 · NCATS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BERNARD, GORDON RAPHAEL · 2012 to 2016
$41.4M
Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ALVIN C POWERS · 2012 to 2026
$29.3M
CLINICAL PHARMACOLOGY TRAINING PROGRAMT32GM007569 · NIGMS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Bjorn C Knollmann · 1985 to 2026
$12.4M
PRIDE: Functional and Translational Genomics of Blood DisordersR25HL106365 · NHLBI · AUGUSTA UNIVERSITY · PI PACE, BETTY SUE · 2010 to 2024
$5.2M
Integrated Training in Engineering and DiabetesT32DK101003 · NIDDK · VANDERBILT UNIVERSITY · PI Jamey D. Young · 2014 to 2026
$3.9M
Determining the contribution of zinc deficiency to perinatal Group B Streptococcus infectionsR01HD090061 · NICHD · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ARONOFF, DAVID M, GADDY, JENNIFER A · 2017 to 2021
$2.5M
The Meharry Research Training InitiativeT32GM144927 · NIGMS · MEHARRY MEDICAL COLLEGE · PI Pamela M Martin · 2022 to 2026
$1.9M
BLRD VA I01 BX005352NCATS NIH HHS UL1 TR000445NCRR NIH HHS UL1 RR024975NHLBI NIH HHS R25 HL106365NICHD NIH HHS R01 HD090061NIDDK NIH HHS P30 DK020593NIDDK NIH HHS T32 DK101003NIGMS NIH HHS T32 GM007569NIGMS NIH HHS T32 GM144927
6 · The paper itself

Abstract

OPA1 is a dynamin-related GTPase that modulates mitochondrial dynamics and cristae integrity. Humans carry eight different isoforms of OPA1 and mice carry five, all of which are expressed as short- or long-form isoforms. These isoforms contribute to OPA1's ability to control mitochondrial energetics and DNA maintenance. However, western blot isolation of all long and short isoforms of OPA1 can be difficult. To address this issue, we developed an optimized western blot protocol based on improving running time to isolate five different isoforms of OPA1 in mouse cells and tissues. This protocol can be applied to study changes in mitochondrial structure and function. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Western Blot Protocol for Isolating OPA1 Isoforms in Mouse Primary Skeletal Muscle Cells.

Indexed as

Blotting, WesternGTP PhosphohydrolasesMuscle, SkeletalAnimalsMiceProtein IsoformsGTP PhosphohydrolasesOpa1 protein, mouseProtein Isoformsisoformsmitochondriamuscle tissueoptic atrophy‐1 (OPA1)western blot

Identifiers

PMID39906927
PMCPMC11795335

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.