ArticleApplications in plant sciences
An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae).
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Seed endophyte Kosakonia cowanii ZX1-2 enhances drought tolerance of Cenchrus pauciflorus via exopolysaccharide-mediated antioxidant and osmotic regulation.BMC plant biology · 2026Article
- Integrated whole-genome resequencing and mRNA-miRNA analysis reveals selection signals and regulatory networks of antipyretic effect variation in Morus alba var. multicaulis.BMC biotechnology · 2026Article
- An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae).Applications in plant sciencesArticle
- Optimizing DNA extraction protocols for bryophytes: Insights from Orthotrichaceae.Applications in plant sciencesArticle
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: Seaweeds are gaining substantial research interest, particularly for genomic applications, where high-quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing protocols often lack versatility, leading to inconsistent outcomes across various materials and taxa, which highlights the need for a universal method for use with a variety of green seaweed samples. Methods and Results: We optimized the conventional cetyltrimethylammonium bromide (CTAB) protocol for green seaweed DNA extraction. Our method, involving an initial sample treatment, lysis buffer adjustment, and enzyme incubation alterations, outperformed the conventional CTAB and commercial kits in terms of DNA yield and purity. Notably, the protocol's effectiveness was demonstrated across various green algal materials and preservation methods, and was tested with downstream applications with satisfactory results. Conclusions: Our optimized CTAB protocol offers a reliable solution for high-quality genomic DNA extraction from a wide variety of green seaweed samples.
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