ArticleFrontiers in cellular and infection microbiology2024
CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms.
Article in Frontiers in cellular and infection microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Antibacterial surface engineering: bioinspiration from leaves to medical devices.RSC applied interfaces · 2026Review
- Emerging Microbiological and Sensor-Based Approaches for Biofilm Detection in Meat and Poultry Processing Environments.Foods (Basel, Switzerland) · 2026Review
- Structure-Dependent Triglyceride Protection of Freeze-DriedFoods (Basel, Switzerland) · 2026Article
- Zinc oxide-bacteriophage synergistic combination for dual-mode antibacterial activity against multidrug-resistant Escherichia coli.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Comparison of Laser and Conventional Root Canal Disinfection Protocols in an dual-biofilm in-vitro model.Lasers in medical science · 2026Article
- Emerging Nanobiochar -Hydrogel Therapeutic Systems: Redox Modulation, Biointerface Interactions, and Critical Gaps in In Vitro Evaluation.Gels (Basel, Switzerland) · 2026Review
- CApEsid biOfilm: a suggested pipeline for clinical phage microbiology for biofilm infections based on comparative method study.Scientific reports · 2026Article
- Phage-antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance.Journal of biomedical science · 2026Article
- Saliva-based molecular diagnostics in oral squamous cell carcinoma (OSCC): a non-invasive frontier in oncology.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2025Review
- The Development of Foodborne Pathogen Detection and Biosensor Design for Surface Plasmon Resonance Technology.Biosensors · 2025Review
- Mastoparan-S from Sphodromantis viridis exhibits antimicrobial activity by disrupting bacterial membranes.AMB Express · 2025Article
- Antimicrobial activity and mechanistic insights of AMP-17 against drug-resistantFrontiers in cellular and infection microbiology · 2025Article
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7 authors.
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Abstract
Introduction: Accurately assessing biofilm viability is essential for evaluating both biofilm formation and the efficacy of antibacterial treatments. Traditional SYTO9 and propidium iodide (PI) live/dead staining in biofilm viability assays often ace challenges due to non-specific staining, limiting precise differentiation between live and dead cells. To address this limitation, we investigated an alternative staining method employing calcein acetoxymethyl (CAM) to detect viable cells based on esterase activity, and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH) to assess the remaining biofilm population. Methods: Biofilms of Results: CAM/TMA-DPH staining demonstrated strong positive correlations with CFU counts across all bacterial species ( Discussion: Our findings suggest that CAM/TMA-DPH staining provides a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms, highlighting the advantages of metabolic-based probes over traditional membrane integrity assays. The consistency of CAM/TMA-DPH staining across different bacterial species underscores its potential to advance studies on biofilm and contribute to the development of more effective anti-biofilm treatments, which is essential for clinical management of biofilm-associated infections.
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