Evidence map›Paper›PMID 39905528›Full record

ArticleEuropean journal of medical research2025

WNT3A promotes the cementogenic differentiation of dental pulp stem cells through the FOXO1 signaling pathway.

Dongmei Cheng, Yang Bao, Xue Wang, Haidong Xiang, Tianyuan Guo, Yong Du, Zhiyong Zhang, Han Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dongmei ChengDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Yang BaoDepartment of Oral Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Xue WangDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Haidong XiangDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Tianyuan GuoDepartment of Plastic Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Yong DuDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Zhiyong ZhangDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Han GuoDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China. 28804419@hebmu.edu.cn.

Funding

Medical Science Research Project in Hebei Province 20241084
6 · The paper itself

Abstract

backgroundDental pulp stem cells (DPSCs) possess capability of multidirectional differentiation, and their cementogenic differentiation potential enables them to participate in cementum repair and regeneration. The molecular mechanisms underlying cementogenic differentiation of DPSCs remain unclear.

methodsDPSC data set GSE138179 was retrieved from gene expression omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was employed to identify significant modules. Pathway enrichment exploration was conducted utilizing gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Set Enrichment Analysis (GSEA), and Metascape tools. CIBERSORT was utilized to analyze immune cell infiltration analysis. The comparative toxicogenomics database (CTD) was utilized for the validation of core targets. Subsequently, cell experiments were conducted to validate the core targets. Changes in protein expression related to the FOXO1 signaling pathway, cell cycle, and apoptosis were evaluated using western blotting (WB).

resultsDifferentially expressed genes (DEGs) associated with DPSC cementogenic differentiation were predominantly enriched in crucial pathways such as the signaling pathway, cell apoptosis, and Wnt signaling pathway. Bioinformatics analysis confirmed WNT3A as a pivotal biomarker for DPSC cementogenic differentiation, and WNT3A was highly expressed in the cementogenic differentiation group. Western blotting results demonstrated that compared to the DPSC group, molecules such as Caspase-3, Caspase-9, FAS, P53, and BAX were downregulated in the CDDPSC group, suggesting reduced apoptosis. Furthermore, upregulation of WNT3A expression in CDDPSC-OE further suppressed the expression of these apoptotic molecules, suggesting a mitigated apoptotic response. Downregulation of WNT3A expression in CDDPSC-KO resulted in increased expression of apoptosis-related molecules, thereby enhancing apoptosis.

conclusionsWNT3A is highly expressed in the cementogenic differentiation of DPSC, and WNT3A mediates FOXO1 pathway to promote differentiation of dental pulp stem cells into cementogenic differentiation, thus realizing the formation and maintenance of cementum tissue.

Indexed as

Cell DifferentiationCementogenesisDental PulpForkhead Box Protein O1Stem CellsWnt3A ProteinApoptosisHumansSignal TransductionWnt Signaling PathwayForkhead Box Protein O1FOXO1 protein, humanWnt3A ProteinWNT3A protein, humanApoptosisCementogenic differentiationDental pulp stem cellsFOXO1WNT3A

Identifiers

PMID39905528
PMCPMC11792666

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.