Evidence map›Paper›PMID 39905410›Full record

ArticleJournal of translational medicine2025

Non-invasive imaging with ICOS-targeting monoclonal antibody for preclinical diagnosis of rheumatoid arthritis in a humanized mouse model.

Shao Duan, Chao Li, Feng Yan, Yifei Xia, Shuaiming Shao, Weiyu Chen, Zunyu Xiao, Gongping Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. ICOS-Targeted Peptide Imaging Informs Therapeutic Response of STING Agonist in Lung Adenocarcinoma.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shao Duan *Department of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Chao Li *Department of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Feng YanDepartment of Orthopedics, the People's Hospital of Suzhou New Distric, Suzhou, China.
Yifei XiaDepartment of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Shuaiming ShaoDepartment of Orthopedics, the People's Hospital of Suzhou New Distric, Suzhou, China.
Weiyu ChenDepartment of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, China. weiyuchen@zju.edu.cn.
Zunyu XiaoDepartment of Nuclear Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, China. fyyxiaozunyu@nbu.edu.cn.
Gongping XuDepartment of Orthopedics, the Second Affiliated Hospital of Harbin Medical University, Harbin, China. 13704846805@163.com.

Funding

Heilongiiang Medical Development Foundation Heilongiiang Medical Development FoundationHeilongiiang Postdoctoral Scientific ResearchDevelopmental Fund LBH-Q21136National Natural Science Foundation of China 82272056National Natural Science Foundation of China 82472036Natural Science Foundation of Heilongiiang Province LH2023H045Ningbo Yongjiang Talent Programme Ningbo Yongjiang Talent ProgrammeResearch Proiect of Suzhou Gusu Health TalentsProgram GSWS2022136The Ningbo Major Research and Development Plan Project 20242214
6 · The paper itself

Abstract

backgroundActivated T cells play a pivotal role in rheumatoid arthritis (RA) pathogenesis, and imaging of activated T cells may provide a non-invasive tool for RA detection. Here, we first developed an optical probe targeting human inducible T cell co-stimulator (ICOS) and tested its capacity in RA diagnosis by capturing ICOS

methodsThe humanized arthritis model, Human peripheral blood mononuclear cells- adjuvant induced arthritis (HuPBMC-AIA) was established, and flow cytometry and immunofluorescence were employed to determine ICOS expression in huPBMC-AIA model. Anti-human ICOS monoclonal antibody (mAb) was conjugated to Cy7 via NHS ester amine reaction. A cell uptake study was used to confirm the specificity of Cy7-ICOS mAb to activated T cells. 4-view near-infrared fluorescence (NIRF) imaging study was performed to test Cy7-ICOS mAb in detecting RA in vivo.

findingsICOS was confirmed as an indicator of RA pathogenesis via RNA-seq, flow cytometry and immunofluorescence data. An in-vitro cellular uptake study validated the specificity of Cy7-ICOS mAb to activated T cells. Cy7-ICOS mAb could detect ICOS

conclusionIn this study, we first developed an optical imaging probe targeting human ICOS, Cy7-ICOS mAb. The 4-view NIRF imaging with Cy7-ICOS mAb could detect pathogenic ICOS

Indexed as

Antibodies, MonoclonalArthritis, RheumatoidInducible T-Cell Co-Stimulator ProteinAnimalsDisease Models, AnimalHumansMiceT-LymphocytesAntibodies, MonoclonalICOS protein, humanInducible T-Cell Co-Stimulator ProteinActivated T cellHumanized modelICOSNIRF imagingRheumatoid arthritis

Identifiers

PMID39905410
PMCPMC11796023

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.