Evidence map›Paper›PMID 39904370›Full record

ArticleOpen biology2025

AGS3-based optogenetic GDI induces GPCR-independent Gβγ signalling and macrophage migration.

Waruna Thotamune, Sithurandi Ubeysinghe, Chathuri Rajarathna, Dinesh Kankanamge, Koshala Olupothage, Aditya Chandu, Bryan A Copits, Ajith Karunarathne

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Waruna Thotamune *Department of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.
Sithurandi Ubeysinghe *Department of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.
Chathuri Rajarathna *Department of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.
Dinesh Kankanamge *Department of Anesthesiology, Washington University Pain Center, Washington University School of Medicine, St. Louis, MO 63110, USA.
Koshala Olupothage *Department of Chemistry and Biochemistry, The University of Toledo, Toledo, OH 43606, USA.
Aditya ChanduDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.
Bryan A CopitsDepartment of Anesthesiology, Washington University Pain Center, Washington University School of Medicine, St. Louis, MO 63110, USA.
Ajith KarunarathneDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.ORCID 0000-0003-3136-2015

Funding

Next Generation Opto-GPCRs for Neuromodulatory ControlU01NS128537 · NINDS · UNIVERSITY OF WASHINGTON · PI Andre Berndt, Michael R. Bruchas · 2023 to 2026
$4.4M
Trans-synaptic optical control of user-defined synaptic connectionsUF1NS133763 · NINDS · WASHINGTON UNIVERSITY · PI COPITS, BRYAN, O'NEILL, PATRICK ROSS · 2023 to 2023
$1.7M
Optical control of endogenous G protein Coupled Receptor and G Protein Signaling.R01GM140191 · NIGMS · UNIVERSITY OF TOLEDO · PI KARUNARATHNE, WELIVITIYA KANKANAMLAGE AJITH · 2021 to 2023
$1.0M
NIGMS NIH HHS R01 GM140191NIH-NIGMSNIH-NINDSNINDS NIH HHS U01 NS128537NINDS NIH HHS UF1 NS133763Saint Louis University
6 · The paper itself

Abstract

G-protein-coupled receptors (GPCRs) are efficient guanine nucleotide exchange factors (GEFs) and exchange GDP to GTP on the Gα subunit of G-protein heterotrimers in response to various extracellular stimuli, including neurotransmitters and light. GPCRs primarily broadcast signals through activated G proteins, GαGTP and free Gβγ and are major disease drivers. Evidence shows that the ambient low threshold signalling required for cells is likely supplemented by signalling regulators such as non-GPCR GEFs and guanine nucleotide dissociation inhibitors (GDIs). Activators of G-protein signalling 3 (AGS3) are recognized as a GDI involved in multiple health and disease-related processes. Nevertheless, understanding of AGS3 is limited, and no significant information is available on its structure-function relationship or signalling regulation in living cells. Here, we employed

Indexed as

GTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsGuanine Nucleotide Dissociation InhibitorsMacrophagesOptogeneticsReceptors, G-Protein-CoupledSignal TransductionAnimalsCell MovementHumansMiceG-protein Beta gammaGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsGuanine Nucleotide Dissociation InhibitorsReceptors, G-Protein-Coupledactivators of G-protein signalling 3GDIGPCRG proteinsmacrophage migrationoptogenetics

Identifiers

PMID39904370
PMCPMC11793977

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.