ArticleFrontiers in chemistry2024
Innovative determination of phytohormones in
Article in Frontiers in chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Anticancer and Antimicrobial Activity ofMolecules (Basel, Switzerland) · 2026Article
- Development and Validation of an LC-MS/MS Method for Quantifying Phytohormones Related to Tomato Shelf Life.Foods (Basel, Switzerland) · 2025Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Aloe vera is widely known for its therapeutic properties, but concerns regarding the levels of phytohormones and their potential impact on human health highlight the need for advanced analytical techniques. This study aims to develop and validate a sensitive method for the determination of six key phytohormones in Aloe vera using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Methods: A validated LC-MS/MS method was optimized for the determination and quantification of six phytohormones in Aloe vera: Abscisic Acid (ABA), Salicylic Acid (SA), Indole-3-Acetic Acid (I3AA), Gibberellic Acid (GA), 6-Benzylaminopurine (6BAP), and Isopentenyladenine (ISA). The sample extraction process and mobile phase composition were optimized to enhance chromatographic separation and mass spectrometry sensitivity. A C-18 column was used for separation, and a triple quadrupole mass spectrometer was employed for quantification. The method's performance was assessed in terms of linearity, sensitivity, and limits of detection. Results: The LC-MS/MS method exhibited excellent linearity ( Discussion: This study provides a comprehensive and validated LC-MS/MS method for profiling phytohormones in Aloe vera. The results underscore the significant role of ABA, I3AA, SA, and GA in the plant's hormonal profile, offering a valuable tool for the analysis of phytohormonal content in Aloe vera and other plant species. The method is particularly beneficial for addressing health-related concerns regarding the presence and concentration of phytohormones in Aloe vera.
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Registered trials
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