Impact of antiplatelet therapy on hemostatic plug formation in the setting of thrombocytopenia.

Background: Antiplatelet therapy (APT), mainly aspirin and P2Y12 receptor inhibitors, reduces the incidence of recurrent arterial thrombosis but also increases bleeding risk. Therefore, management of APT in patients with thrombocytopenia, itself an independent risk factor for bleeding, is a clinical challenge with few evidence-based guidelines. Data are lacking on the combined impact of thrombocytopenia and APT on hemostasis. Objectives: To systematically investigate the combined effect of thrombocytopenia and APT in mouse models of hemostasis and thrombosis. Methods: Platelet-depleted mice were repleted with donor platelets inhibited with aspirin and/or clopidogrel at low (<1 × 10 Results: In the saphenous vein laser injury model, neither single nor dual APT significantly increased bleeding compared with vehicle at platelet counts >2 × 10 Conclusion: Our experimental data suggests that for severe thrombocytopenia, single APT provides an appropriate balance of antithrombotic effect and limited bleeding, with clopidogrel demonstrating a greater antithrombotic effect but slightly increased bleeding compared with aspirin.