Evidence map›Paper›PMID 39899352›Full record

ArticleeLife2025

Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo.

Taro Ichimura, Taishi Kakizuka, Yoshitsugu Taniguchi, Satoshi Ejima, Yuki Sato, Keiko Itano, Kaoru Seiriki, Hitoshi Hashimoto, Ko Sugawara, Hiroya Itoga and 2 more

Abstract read
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Self-generated chemotaxis of mixed cell populations.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Article
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Taro IchimuraTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan.ORCID https://orcid.org/0000-0002-3740-3634
Taishi KakizukaTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan.
Yoshitsugu TaniguchiSIGMAKOKI CO LTD, Midori, Sumida-ku, Tokyo, Japan.
Satoshi EjimaSIGMAKOKI CO LTD, Midori, Sumida-ku, Tokyo, Japan.
Yuki SatoDepartment of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Keiko ItanoDepartment of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, Japan.
Kaoru SeirikiLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Hitoshi HashimotoTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan.ORCID https://orcid.org/0000-0001-6548-4016
Ko SugawaraLaboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
Hiroya ItogaLaboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
Shuichi OnamiTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan.ORCID https://orcid.org/0000-0002-8255-1724
Takeharu NagaiTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan.

Funding

Japan Agency for Medical Research and Development JP21dm0207117Japan Agency for Medical Research and Development JP23ama121052Japan Agency for Medical Research and Development JP23ama121054Japan Science and Technology Agency 10.52926/jpmjcr15n3Japan Science and Technology Agency 10.52926/jpmjcr1926Japan Science and Technology Agency 10.52926/jpmjnd2201Japan Science and Technology Agency 10.52926/JPMJPR18G2Japan Society for the Promotion of Science 18H05408Japan Society for the Promotion of Science 18H05410Japan Society for the Promotion of Science 18H05412Japan Society for the Promotion of Science 18H05416Japan Society for the Promotion of Science 21H00431Japan Society for the Promotion of Science 21H05590Japan Society for the Promotion of Science 23H041350Japan Society for the Promotion of Science JP23H00395Japan Society for the Promotion of Science JP24K22022
6 · The paper itself

Abstract

We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm

Indexed as

BrainEmbryo, MammalianImaging, Three-DimensionalOptical ImagingAnimalsEmbryo, NonmammalianMiceQuailTime-Lapse Imagingdevelopmental biologyhumanmouseneuroscienceoptical microscopyquailtrans-scale imaging

Identifiers

PMID39899352
PMCPMC11790249

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.