Evidence map›Paper›PMID 39898784›Full record

ArticleClinical and experimental dental research2025

Differential Expression of Hard Tissue Proteins in Hypomineralized Second Primary Molars in Comparison to Normal Teeth.

Sharon Jessica, Ramya Sekar, Snehashish Ghosh, Safal Dhungel, Kavitha B, Mahesh Ramakrishnan, Shazia Fathima Jh, Monisha Prasad, Jaiganesh I, Sindhu Subramani

Abstract readComparative Study
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Article in Clinical and experimental dental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

10 authors.

Sharon JessicaDepartment of Oral and Maxillofacial Pathology and Oral Microbiology, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India.
Ramya SekarDepartment of Oral and Maxillofacial Pathology and Oral Microbiology, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India.
Snehashish GhoshDepartment of Oral Pathology, College of Medical Sciences, Bharatpur, Nepal.ORCID 0000-0002-7349-1619
Safal DhungelDepartment of Oral and Maxillofacial Surgery, College of Medical Sciences, Bharatpur, Nepal.
Kavitha BDepartment of Oral and Maxillofacial Pathology and Oral Microbiology, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India.
Mahesh RamakrishnanDepartment of Pedodontics & Preventive Dentistry, Saveetha Dental College & Hospital, Saveetha Institute of Medical & Technical Sciences, Chennai, Tamil Nadu, India.
Shazia Fathima JhDepartment of Biochemistry, Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College & Hospital, Saveetha Institute of Medical & Technical Sciences, Chennai, Tamil Nadu, India.
Monisha PrasadCentre for Global Health Research, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.
Jaiganesh IDepartment of Pediatric & Preventive Dentistry, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India.
Sindhu SubramaniDepartment of Oral and Maxillofacial Pathology and Oral Microbiology, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India.

Funding

The authors received no specific funding for this work.
6 · The paper itself

Abstract

objectiveThis study aims to identify the proteins in hypomineralized second primary molars (HSPMs) and correlate their function in Amelogenesis. HSPM is a qualitative defect of the enamel of the second primary molars with no clear etiology. MATERIAL AND

methodsTotal protein quantification was performed using the Bradford Protein Assay, followed by the electrophoretic separation of samples using 2D-Gel electrophoresis to identify the proteins.

resultsThe results from the Bradford Protein Assay unveiled a five-fold increase in the protein content in HSPM. Proteins such as Dentin sialo-phosphoprotein (DSPP), Keratin, type I, Serum Albumin, Anti-thrombin III, Alpha-1-Antitrypsin, Histone H3.2, Actin, Heat shock Protein, Vimentin, Desmoglein-3, Glyceraldehyde-3-phosphate dehydrogenase, Inosine-5'-monophosphate dehydrogenase 2, Zinc Alpha 2 glycoprotein, Lysozyme C, Prothrombin, Vit-D binding Protein, Apolipoprotein A-1, Defensin 1, Immunoglobulin Gamma, Immunoglobulin Kappa, and Alpha-Amylase were all upregulated (p < 0.05) in HSPM.

conclusionThis investigation conclusively demonstrates that HSPM-affected teeth have higher protein content than healthy teeth. The study also supports the theory of proteolytic inhibition attributed to reduced protease activity and heightened protease inhibitor activity.

Indexed as

Dental Enamel ProteinsMolarTooth, DeciduousAmelogenesisChildElectrophoresis, Gel, Two-DimensionalHumansDental Enamel Proteinsdental carieshypomineralizationmolarsprimary teeth

Identifiers

PMID39898784
PMCPMC11789269

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