Evidence map›Paper›PMID 39896675›Full record

ArticlebioRxiv : the preprint server for biology2025

Enantioselective Protein Affinity Selection Mass Spectrometry (E-ASMS).

Xiaoyun Wang, Jianxian Sun, Shabbir Ahmad, Diwen Yang, Fengling Li, U Hang Chan, Hong Zeng, Conrad V Simoben, Stuart R Green, Madhushika Silva and 28 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

38 authors.

Xiaoyun WangDepartment of Chemistry, University of Toronto, Toronto, ON, Canada.
Jianxian SunDepartment of Chemistry, University of Toronto, Toronto, ON, Canada.ORCID 0000-0002-0385-9959
Shabbir AhmadStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Diwen YangDepartment of Physical & Environmental Sciences, University of Toronto Scarborough, Toronto ON, Canada.
Fengling LiStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
U Hang ChanStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Hong ZengStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Conrad V SimobenStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Stuart R GreenStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Madhushika SilvaStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Scott HoulistonPrincess Margaret Cancer Centre, University of Toronto, Toronto, ON, Canada.
Aiping DongStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Albina BolotokovaStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Elisa GibsonStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Maria KuteraStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Pegah GhiabiStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Ivan KondratovEnamine Ltd., Winston Churchill Street 78, 02094 Kyiv, Ukraine.
Tetiana MatviyukEnamine Ltd., Winston Churchill Street 78, 02094 Kyiv, Ukraine.
Alexander ChuprinaEnamine Ltd., Winston Churchill Street 78, 02094 Kyiv, Ukraine.
Danai MavridiInstitute of Pharmaceutical Chemistry, Goethe University, Frankfurt am Main, Germany.
Christopher LenzInstitute of Pharmaceutical Chemistry, Goethe University, Frankfurt am Main, Germany.
Andreas C JoergerInstitute of Pharmaceutical Chemistry, Goethe University, Frankfurt am Main, Germany.
Benjamin D BrownBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
Richard B HeathBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
Wyatt W YueBiosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
Lucy K RobbieEmory University School of Medicine, Atlanta, GA, USA.
Tyler S BeyettEmory University School of Medicine, Atlanta, GA, USA.ORCID 0000-0001-5509-7004
Susanne MüllerInstitute of Pharmaceutical Chemistry, Goethe University, Frankfurt am Main, Germany.
Stefan KnappInstitute of Pharmaceutical Chemistry, Goethe University, Frankfurt am Main, Germany.
Rachel HardingStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Matthieu SchapiraStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.ORCID 0000-0002-1047-3309
Peter J BrownStructural Genomics Consortium, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Vijayaratnam SanthakumarStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Suzanne AcklooStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Cheryl H ArrowsmithStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Aled M EdwardsStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Hui PengDepartment of Chemistry, University of Toronto, Toronto, ON, Canada.
Levon HalabelianStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.

Funding

TREAT AD Structural Biology CoreU54AG065187 · NIA · EMORY UNIVERSITY · PI Gregory W Carter, ALLAN I LEVEY · 2019 to 2026
$69.7M
NIA NIH HHS U54 AG065187
6 · The paper itself

Abstract

We report an enantioselective protein affinity selection mass spectrometry screening approach (E-ASMS) that enables the detection of weak binders, informs on selectivity, and generates orthogonal confirmation of binding. After method development with control proteins, we screened 31 human proteins against a designed library of 8,210 chiral compounds. 16 binders to 12 targets, including many proteins predicted to be "challenging to ligand", were discovered and confirmed in orthogonal biophysical assays. 7 binders to 6 targets bound in an enantioselective manner, with

Identifiers

PMID39896675
PMCPMC11785093

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.