Evidence map›Paper›PMID 39894194›Full record

ArticleToxicology2025

Comparing the cannabidiol-induced transcriptomic profiles in human and mouse Sertoli cells.

Yuxi Li, Xilin Li, Patrick Cournoyer, Supratim Choudhuri, Lei Guo, Si Chen

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuxi LiDivision of Biochemical Toxicology, National Center for Toxicological Research, U S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA.
Xilin LiDivision of Genetic and Molecular Toxicology, National Center for Toxicological Research, U S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA.
Patrick CournoyerOffice of the Commissioner, U S. Food and Drug Administration, Silver Spring, MD 20993, USA.
Supratim ChoudhuriDivision of Food Ingredients, Office of Premarket Additive Safety, Office of Food Chemical Safety, Dietary Supplements, and Innovation, Human Foods Program, U S. Food and Drug Administration, College Park, MD 20740, USA.
Lei GuoDivision of Biochemical Toxicology, National Center for Toxicological Research, U S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA.
Si ChenDivision of Biochemical Toxicology, National Center for Toxicological Research, U S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA. Electronic address: Si.Chen@fda.hhs.gov.

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

Cannabidiol (CBD), a major cannabinoid found in Cannabis sativa L., has been used in the treatment of seizures associated with Lennox-Gastaut syndrome, Dravet syndrome, and tuberous sclerosis complex. Recently, concerns have been raised regarding the male reproductive toxicity of CBD in animal models, such as monkeys, rats, and mice. In our previous studies, we reported that CBD inhibited cell proliferation in both primary human Sertoli cells and mouse Sertoli TM4 cells. Transcriptomic analysis revealed that in primary human Sertoli cells CBD disrupted DNA replication, cell cycle, and DNA repair, ultimately causing cellular senescence. In this study, we further investigated the molecular changes induced by CBD in mouse Sertoli TM4 cells using RNA-sequencing analyses and compared the transcriptomic profile with that of primary human Sertoli cells. Our findings demonstrated that, unlike in primary human Sertoli cells, CBD did not induce cellular senescence but caused apoptosis in mouse Sertoli TM4 cells. Through transcriptomic data analysis in mouse Sertoli TM4 cells, immune and cellular stress responses were identified. Moreover, transcriptomic comparisons revealed major differences in molecular changes induced by CBD between mouse Sertoli TM4 and primary human Sertoli cells. This suggests that primary human Sertoli cells and mouse Sertoli cells may respond differently to CBD.

Indexed as

CannabidiolSertoli CellsTranscriptomeAnimalsApoptosisCells, CulturedCellular SenescenceGene Expression ProfilingHumansMaleMiceMice, Inbred C57BLCannabidiolApoptosisCannabidiolMale reproductive toxicityMouse Sertoli TM4 cellsmRNA-sequencingPrimary human Sertoli cells

Identifiers

PMID39894194
PMCPMC12376089

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.