Evidence map›Paper›PMID 39893722›Full record

ArticleFood chemistry2025

Unraveling the trisubstrate-triproduct reaction mechanisms of wine grape VvCYP76F14 to improve wine bouquet.

Zhizhong Song, Meiling Tang, Huilin Xiao, Houhua Xu, Matthew Shi, Adeeba Dark, Zhenqiang Xie, Bin Peng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhizhong SongJiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212499, China; Department of Plant Science, University of Cambridge, Cambridge CB2 3EA, UK; College of Horticulture, Ludong University/Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Yantai 264025, China.
Meiling TangCollege of Horticulture, Ludong University/Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Yantai 264025, China; Yantai Academy of Agricultural Sciences, Yantai 265599, China; Shandong Technology Innovation Center of Wine Grape and Wine/ COFCO Great Wall Wine (Penglai) Co., Ltd, Yantai, 264000, China.
Huilin XiaoCollege of Horticulture, Ludong University/Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Yantai 264025, China; Yantai Academy of Agricultural Sciences, Yantai 265599, China; Shandong Technology Innovation Center of Wine Grape and Wine/ COFCO Great Wall Wine (Penglai) Co., Ltd, Yantai, 264000, China.
Houhua XuCollege of Horticulture, Ludong University/Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Yantai 264025, China; Shandong Technology Innovation Center of Wine Grape and Wine/ COFCO Great Wall Wine (Penglai) Co., Ltd, Yantai, 264000, China.
Matthew ShiDepartment of Plant Science, University of Cambridge, Cambridge CB2 3EA, UK; College of Horticulture, Ludong University/Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Yantai 264025, China.
Adeeba DarkDepartment of Plant Science, University of Cambridge, Cambridge CB2 3EA, UK.
Zhenqiang XieJiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212499, China; Shandong Technology Innovation Center of Wine Grape and Wine/ COFCO Great Wall Wine (Penglai) Co., Ltd, Yantai, 264000, China; Cocodala Vocational and Technical College, Cocodala 853213, China. Electronic address: xiezhenqiang@jsafc.edu.cn.
Bin PengJiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212499, China; College of Horticulture, Ludong University/Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Yantai 264025, China; Cocodala Vocational and Technical College, Cocodala 853213, China. Electronic address: pengbin@jsafc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bouquet is a captivating wine characteristic that affects consumer consumption. Wine lactone correlates with both precursor ((E)-8-carboxylinalool) concentration and wine age that has the greatest contribution to bouquet. In wine grape, cytochrome P450 VvCYP76F14 can catalyze trisubstrate-triproduct reaction processes (hydroxylation, dehydrogenation and carboxylation) to produce (E)-8-carboxylinalool. However, the exact mechanism of the whole reaction is unclear. Here, we unraveled the multi-catalysis mechanism with the aim of rapid conversion of linalool into (E)-8-carboxylinalool in winemaking. Results showed that the redox partner NADPH cytochrome P450 reductase (VvCPR1) was indispensable for hydroxylation and carboxylation, but not dehydrogenation oxidation. Furthermore, the VvCYP76F14-VvCPR1 complex was introduced in the aging stage of winemaking and results showed that the complex could improve the bouquet by increasing the content of wine lactone and shortening the aging time. Nonetheless, this study reveals the trisubstrate-triproduct reaction mechanism of VvCYP76F14 and the VvCYP76F14/VvCPR1 complex has the potential use for wine bouquet enrichment.

Indexed as

Cytochrome P-450 Enzyme SystemPlant ProteinsVitisWineAcyclic MonoterpenesHydroxylationLactonesOxidation-ReductionAcyclic MonoterpenesCytochrome P-450 Enzyme SystemLactoneslinaloolPlant ProteinsCatalytic mechanismsCytochrome P450 enzymeTrisubstrate-triproductWine bouquetWine grape

Identifiers

PMID39893722
PMCPMC11877275

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.