Evidence map›Paper›PMID 39893476›Full record

ArticleBMC microbiology2025

Xanthomonas citri subsp. citri requires a polyketide cyclase to activate the type III secretion system for virulence.

Shuying Zhu, Siyu Wu, Yanmin Liu, Zaibao Zhang, Huasong Zou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Pathogens (Basel, Switzerland) · 2026
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shuying ZhuSchool of Life and Health Sciences, Huzhou College, Huzhou, 313000, Zhejiang, China.
Siyu WuSchool of Life and Health Sciences, Huzhou College, Huzhou, 313000, Zhejiang, China.
Yanmin LiuSchool of Life and Health Sciences, Huzhou College, Huzhou, 313000, Zhejiang, China.
Zaibao ZhangSchool of Life and Health Sciences, Huzhou College, Huzhou, 313000, Zhejiang, China.
Huasong ZouSchool of Life and Health Sciences, Huzhou College, Huzhou, 313000, Zhejiang, China. zouhuasong@zjhzu.edu.cn.

Funding

the National Natural Science Foundation of China 31872919
6 · The paper itself

Abstract

backgroundXanthomonas citri subsp. citri is the causal agent of citrus canker, which causes substantial losses in citrus production. Here, we report the role of a polyketide cyclase (PKC) on the virulence in X. citri subsp. citri.

methodsThe structure of PKC was precisely predicted using Alphafold3. Promoter GUS fusion constructs and real-time quantitative reverse transcription (qRT-PCR) were employed to study the pattern of expression of the polyketide gene. A deletion mutation was created to explore the role of PKC in virulence and metabolic change.

resultsThe PKC was determined to have a signal peptide, a START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC (SRPBCC) domain, and a GyrI-like small molecule binding domain. The expression of the PKC gene was induced in planta, as well as under stress by CuSO

conclusionsThe overall data indicate that the PKC affects bacterial virulence by modulating the type III secretion system, possibly through the biosynthesis of particular metabolites.

Indexed as

Bacterial ProteinsPolyketide SynthasesType III Secretion SystemsXanthomonasCitrusGene Expression Regulation, BacterialPlant DiseasesVirulenceBacterial ProteinsPolyketide SynthasesType III Secretion SystemsCitrus cankerPolyketide cyclaseType III geneVirulenceXanthomonas citri subsp. citri

Identifiers

PMID39893476
PMCPMC11786466

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.