Evidence map›Paper›PMID 39893283›Full record

ArticleMikrochimica acta2025

Rapid and facile detection of Mycoplasma pneumoniae 16S rRNA based on CHA-FICA system.

Shijie Cai, Yuming Yao, Yaya Chen, Gulinaizhaer Abudushalamua, Shuo Ma, Jiwei Wang, Chen Zhang, Xun Gao, Guoqiu Wu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Research progress on detection technologies for Mycoplasma pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shijie CaiCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Yuming YaoCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Yaya ChenCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Gulinaizhaer AbudushalamuaCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Shuo MaCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Jiwei WangCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Chen ZhangCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China.
Xun GaoCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China. gaoxun1021@163.com.
Guoqiu WuCenter of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, People's Republic of China. nationball@163.com.

Funding

Jiangsu Provincial Key Laboratory of Critical Care Medicine JSKLCCM202202015Jiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit JSDW202240National Natural Science Foundation of China 82302609National Natural Science Foundation of China 82373781Natural Science Foundation of Jiangsu Province BK20230840Research Personnel Cultivation Program of Zhongda Hospital, Southeast University CZXM-GSP-RC97Special Funds for Research in Laboratory Medicine, Jiangsu Medical Association SYH-3201160-0054 (2023001)Tang Scholar supporting grant 3290002409A2
6 · The paper itself

Abstract

Mycoplasma pneumoniae (MP) is a major a rapid, sensitive and reliable method for the detection of 16S ribosomal RNA (16S rRNA) was developed based on catalytic hairpin assembly (CHA) technology and digoxine-biotin double-labeled fluorescence immunochromatography (FICA) strip. The signal amplification achieved by CHA avoids the dependence on expensive thermal cycling equipment. The method can obtain the results within 1 h in the absence of an enzyme, and the minimum detection limit can reach 1 pM. It has good specificity and can effectively distinguish target sequences from non-target sequences. After verification with adult samples and pediatric samples, it was found that CHA-FICA detection was highly consistent with those of PCR detection, indicating that it has clinical application potential and is expected to be used in clinical screening and treatment detection of mycoplasma pneumoniae (MP) infection.

Indexed as

Chromatography, AffinityMycoplasma pneumoniaeRNA, Ribosomal, 16SCatalysisHumansLimit of DetectionPneumonia, MycoplasmaRNA, Ribosomal, 16SCatalyzed hairpin assemblyFluorescence immunochromatography assayMycoplasma pneumoniae

Identifiers

PMID39893283

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.