Evidence map›Paper›PMID 39891706›Full record

ArticleInternational journal of legal medicine2025

Integration of a high-resolution melt curve assay into a commercial quantification kit for preliminary identification of biological mixtures.

Chastyn Smith, Andrea L Williams, Hannah E Wines, Darianne C Cloudy, Jordan O Cox, Sarah J Seashols-Williams, Edward L Boone, Tracey Dawson Green

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of legal medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chastyn SmithDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA. smithc13@vcu.edu.ORCID http://orcid.org/0000-0003-1353-6034
Andrea L WilliamsDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.
Hannah E WinesDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.
Darianne C CloudyDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.ORCID http://orcid.org/0009-0008-1125-6622
Jordan O CoxDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.ORCID http://orcid.org/0000-0003-4483-9185
Sarah J Seashols-WilliamsDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.ORCID http://orcid.org/0000-0003-0093-8261
Edward L BooneDepartment of Statistical Sciences & Operations Research, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.ORCID http://orcid.org/0000-0003-0755-6899
Tracey Dawson GreenDepartment of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.ORCID http://orcid.org/0000-0003-4761-6657

Funding

National Institute of Justice 2019-DU-BX-0003
6 · The paper itself

Abstract

In recent years, DNA analysis techniques have drastically increased in sensitivity, allowing for low template DNA samples to be more easily detected and used for identification. Since the problems inherent in low template DNA are exacerbated in DNA mixture samples, it would be advantageous to incorporate an assay earlier in the DNA workflow that could detect a mixture and, potentially, determine the number of contributors. Some real-time PCR instruments have high-resolution melt curve analysis (HRM) capabilities, allowing for an opportunity to integrate an HRM screening assay into a commercial DNA quantification kit. This work describes the integration of a mixture screening HRM assay using STR loci D5S818 and D18S51 into Qiagen's Investigator Quantiplex

Indexed as

DNADNA FingerprintingMicrosatellite RepeatsReal-Time Polymerase Chain ReactionTransition TemperatureDiscriminant AnalysisHumansSupport Vector MachineDNAForensic scienceHigh-resolution melt curve (HRM)Investigator Quantiplex® kitLinear discriminant analysisMixture screeningSupport vector machine modeling (SVM)

Identifiers

PMID39891706
PMCPMC12003509

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.