SynthesisScientific reports2025
Best practices for cryopreserving sperm in Nonhuman Primates: a systematic review and meta-analysis.
Synthesis in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Proteomic Profiling of Cryopreservation-Induced Alterations in Rhesus Macaque Sperm.Andrology · 2026Article
- Recovery of high-quality sperm after cryopreservation in the common marmoset (Callithrix jacchus)†.Biology of reproduction · 2026Article
- Impact of cryopreservation agents on sperm quality, DNA fragmentation, and apoptotic markers in fertile and infertile males.Scientific reports · 2025Article
- Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Nonhuman primates (NHPs) are in increasing demand as models for preclinical research. The availability of a highly efficient cryopreservation protocol for use with NHP sperm has potential to significantly alleviate this demand and contribute to rapid increases in overall number of NHPs available for biomedical research. Thus, this systematic review assesses the state-of-the-art in NHP sperm cryopreservation and uses meta-analyses to describe the impact of varied cryopreservation approaches on NHP sperm. We searched the literature deposited in PubMed, Scopus, and Web of Science databases through June 2024 to identify data relevant to the effect of sperm cryopreservation on quality of thawed sperm compared to fresh sperm across NHP species. All original NHP studies reporting sperm parameters both before cryopreservation and after thawing were included for analysis. In total, 32 articles were included for qualitative analysis and not surprisingly, all 32 studies demonstrated negative effects of cryopreservation on sperm parameters, Specifically, motility and viability were significantly decreased in thawed NHP sperm. While the method of sperm collection did not have a significant impact on recovery of motile sperm, significant variability was evident between species. Importantly, recovery of motile NHP sperm was greatest following rapid sperm freezing methods. This analysis provides critical insights into future research directions to enhance and optimize cryopreservation protocols in NHPs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.