Evidence map›Paper›PMID 39890805›Full record

ArticleScientific reports2025

Systematic study on the evaluation method of surface antibacterial activity based on the fluorescent observation of bacterial growth.

Noriko Nakamura, Tatsuki Yamagishi, Wataru Nagata, Yuki Akahane, Hyemin Seo, Ayaka Otsuka, Daiki Hishikawa, Risa Yoshimoto, Nobuhiro Miyamae, Seiichi Ohta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Noriko NakamuraInstitute of Engineering Innovation, University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo, 113-8656, Japan.
Tatsuki YamagishiDepartment of Bioengineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113- 8656, Japan.
Wataru NagataDepartment of Chemical System Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Yuki AkahaneDepartment of Chemical System Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Hyemin SeoDepartment of Bioengineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113- 8656, Japan.
Ayaka OtsukaDepartment of Bioengineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113- 8656, Japan.
Daiki HishikawaNippon Paint Co., Ltd, 4-1-15 Minamishinagawa, Shinagawa-ku, Tokyo, 140-8675, Japan.
Risa YoshimotoNippon Paint Co., Ltd, 4-1-15 Minamishinagawa, Shinagawa-ku, Tokyo, 140-8675, Japan.
Nobuhiro MiyamaeNippon Paint Co., Ltd, 4-1-15 Minamishinagawa, Shinagawa-ku, Tokyo, 140-8675, Japan.
Seiichi OhtaInstitute of Engineering Innovation, University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo, 113-8656, Japan. s-ohta@sogo.t.u-tokyo.ac.jp.

Funding

Japan Science and Technology Agency JPMJPR20A1Japan Society for the Promotion of Science 21H01722Japan Society for the Promotion of Science 21K20478
6 · The paper itself

Abstract

Antibacterial and antiviral coating materials have attracted increasing attention for the prevention of infections caused by frequently touched surfaces in communities and hospitals. The standard assessment procedure for antibacterial surfaces involves bacterial culture on a film-covered substrate followed by transfer onto agar for colony counting (ISO22196:2011). However, this assessment lacks temporal and spatial information regarding bacterial growth, resulting in an incomplete and inaccurate evaluation of the antibacterial activity of the surface. In this study, we develop a novel evaluation procedure for antibacterial substrates that enables in situ visualization of bacterial growth on a surface with centimeter-scale spatial information using fluorescent protein-expressing bacterial cells and an image acquisition setup. The effects of equipment parameters on bacterial growth are systematically investigated to establish the standard evaluation conditions. Based on the optimized parameters, a quantitative evaluation of the antibacterial activity of the coating material is successfully demonstrated. The proposed method is expected to be useful in investigating the spatial and temporal distribution of bacterial growth on substrates.

Indexed as

Anti-Bacterial AgentsBacteriaEscherichia coliMicrobial Sensitivity TestsSurface PropertiesAnti-Bacterial AgentsAntibacterial activityBacterial growthFluorescent observationin situ visualization

Identifiers

PMID39890805
PMCPMC11785733

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.