Evidence map›Paper›PMID 39890715›Full record

ArticleInternational ophthalmology2025

Diurnal rhythm-modulated transcriptome analysis of meibomian gland in hyperlipidemic mice using RNA sequencing.

Qing Zhang, Jinxing Su, Jing Chen, Sainan Wu, Xiaoxuan Qi, Min Chu, Shangquan Jiang, Kan He

Abstract read
PubMed Publisher
In one paragraph

Article in International ophthalmology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qing ZhangDepartment of Ophthalmology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, 230601, Anhui, People's Republic of China. zhangqing@ahmu.edu.cn.
Jinxing SuCenter for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, 230600, Anhui, People's Republic of China.
Jing ChenDepartment of Ophthalmology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, 230601, Anhui, People's Republic of China.
Sainan WuDepartment of Ophthalmology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, 230601, Anhui, People's Republic of China.
Xiaoxuan QiDepartment of Ophthalmology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, 230601, Anhui, People's Republic of China.
Min ChuCenter for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, 230600, Anhui, People's Republic of China.
Shangquan JiangCenter for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, 230600, Anhui, People's Republic of China.
Kan HeCenter for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, 230600, Anhui, People's Republic of China. hekan_803@163.com.

Funding

Key Projects of Natural Science Research in Colleges and Universities of Anhui Province KJ2020A0180Natural Science Foundation of Anhui Province 1908085MC87Overseas Visiting and Study Program for Outstanding Young Backbone Talents of Colleges and Universities of Anhui Province gxgwfx2020024Science and Technology New Star Training Project of Anhui Medical University 2018KA08Scientific Research Foundation and Academic & Technology Leaders Introduction Project, and 211 Project of Anhui University 10117700023Second Hospital of Anhui Medical University Incubation Project of NSFC 2019GQFY04
6 · The paper itself

Abstract

aimTo explore the regulatory mechanism of meibomian gland (MG) in hyperlipidemic mice under a diurnal rhythm by transcriptomic analysis based on high-throughput sequencing.

methodsThe mouse model of hyperlipidemia induced by four months of high-fat diet (HFD) feeding to a regular light-dark (LD) cycle for 2 weeks was used in this study. Phenotypic observation and RNA sequencing (RNA-seq) of MGs of the experimental mice were then performed to investigate transcriptional changes due to hyperlipidemia and the diurnal rhythm and their effects on meibomian gland dysfunction (MGD).

resultsThe expression levels of the identified dysregulated genes were then validated by qRT-PCR. Several significantly regulated genes and enriched pathways were identified as associated with MGD in hyperlipidemic mice under a diurnal rhythm; these genes included some core diurnal clock genes, e.g., Clock, Per2 and Per3. Phenotypic and histological analysis reveals abnormal morphology concomitantly with a modification of the transcriptional landscape of MG caused by HFD.

conclusionOur findings provide us with a deeper understanding of the diurnal rhythm regulation of MG in hyperlipidemic mice altered by daily nutritional challenge.

Indexed as

Circadian RhythmGene Expression ProfilingGene Expression RegulationHyperlipidemiasMeibomian Gland DysfunctionMeibomian GlandsTranscriptomeAnimalsDiet, High-FatDisease Models, AnimalMaleMiceMice, Inbred C57BLSequence Analysis, RNADiurnal rhythmHyperlipidemic miceMeibomian glandTranscriptomics

Identifiers

PMID39890715

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.