Evidence map›Paper›PMID 39890457›Full record

ArticleeNeuro2025

A Simple, Low-Cost Implant for Reliable Diaphragm EMG Recordings in Awake, Behaving Rats.

Taylor C Holmes, Jesus D Penaloza-Aponte, Alyssa R Mickle, Rachel L Nosacka, Erica A Dale, Kristi A Streeter

Abstract read
In one paragraph

Article in eNeuro, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Taylor C HolmesExercise and Rehabilitation Science Program, Department of Physical Therapy, Marquette University, Milwaukee, Wisconsin 53233.
Jesus D Penaloza-AponteDepartment of Neuroscience, University of Florida, Gainesville, Florida 32610.ORCID https://orcid.org/0000-0001-5903-2291
Alyssa R MickleDepartment of Neuroscience, University of Florida, Gainesville, Florida 32610.
Rachel L NosackaDepartments of Pediatrics, University of Florida, Gainesville, Florida 32610.
Erica A DaleDepartment of Neuroscience, University of Florida, Gainesville, Florida 32610.ORCID https://orcid.org/0000-0002-9909-8858
Kristi A StreeterExercise and Rehabilitation Science Program, Department of Physical Therapy, Marquette University, Milwaukee, Wisconsin 53233 kristi.streeter@marquette.edu.

Funding

Clinical and Translational Science AwardUL1TR001436 · NCATS · MEDICAL COLLEGE OF WISCONSIN · PI FREED, JULIE K · 2015 to 2025
$47.5M
Functional mapping of peripheral and central circuits for airway protection and breathingOT2OD023854 · OD · UNIVERSITY OF FLORIDA · PI BOLSER, DONALD C · 2016 to 2020
$10.8M
J. NRSA Training CoreTL1TR001437 · NCATS · MEDICAL COLLEGE OF WISCONSIN · PI THOMAS, DANNY GEORGE · 2015 to 2024
$3.7M
Breathing Research and Therapeutics (BREATHE)T32HL134621 · NHLBI · UNIVERSITY OF FLORIDA · PI Gordon S. Mitchell · 2017 to 2026
$3.4M
Cervical Epidural Stimulation and Respiratory Motor PlasticityR01HL153102 · NHLBI · UNIVERSITY OF FLORIDA · PI DALE, ERICA ARDEN · 2021 to 2025
$2.0M
Phrenic Afferents and Neuromuscular Plasticity Following Spinal Cord InjuryR00HL143207 · NHLBI · MARQUETTE UNIVERSITY · PI STREETER, KRISTI ANN · 2020 to 2022
$732k
NCATS NIH HHS TL1 TR001437NCATS NIH HHS UL1 TR001436NHLBI NIH HHS R00 HL143207NHLBI NIH HHS R01 HL153102NHLBI NIH HHS T32 HL134621NIH HHS OT2 OD023854
6 · The paper itself

Abstract

Breathing is a complex neuromuscular process vital to sustain life. In preclinical animal models, the study of respiratory motor control is primarily accomplished through neurophysiologic recordings and functional measurements of respiratory output. Neurophysiologic recordings that target neural or muscular output via direct nerve recordings or respiratory muscle electromyography (EMG) are commonly collected during anesthetized conditions. While offering tight control of experimental preparations, the use of anesthesia results in respiratory depression, may impact cardiovascular control, eliminates the potential to record volitional nonventilatory behaviors, and can limit translation. Since the diaphragm is a unique muscle which is rhythmically active and difficult to access, placing diaphragm EMGs to collect chronic recordings in awake animals is technically challenging. Here, we describe methods for fabricating and implanting indwelling diaphragm EMG electrodes to enable recordings from awake rodents for longitudinal studies. These electrodes are relatively easy and quick to produce (∼1 h), are affordable, and provide high-quality and reproducible diaphragm signals using a tethered system that allows animals to

Indexed as

DiaphragmElectrodes, ImplantedElectromyographyWakefulnessAnimalsMaleRatsRats, Sprague-Dawleybreathingchronic recordingsdiaphragmelectromyographyrespiratory muscle

Identifiers

PMID39890457
PMCPMC11839091

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.