ArticleThe Journal of cell biology1985
Role of myosin in terminal web contraction in isolated intestinal epithelial brush borders.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- A multicellular star-shaped actin network underpins epithelial organization and connectivity.Nature communications · 2025Article
- Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli.Molecular biology of the cell · 2020Article
- Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules.Biophysical journal · 2019Article
- Plasticity of the brush border - the yin and yang of intestinal homeostasis.Nature reviews. Gastroenterology & hepatology · 2016Review
- Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-γ.The American journal of pathology · 2014Article
- The minus-end actin capping protein, UNC-94/tropomodulin, regulates development of the Caenorhabditis elegans intestine.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Non-lytic, actin-based exit of intracellular parasites from C. elegans intestinal cells.PLoS pathogens · 2011Article
- A novel terminal web-like structure in cortical lens fibers: architecture and functional assessment.Anatomical record (Hoboken, N.J. : 2007) · 2010Article
- Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium.Molecular biology of the cell · 2009Article
- Diffusible retinal secretions regulate the expression of tight junctions and other diverse functions of the retinal pigment epithelium.Molecular vision · 2008Article
- Myosin-1a powers the sliding of apical membrane along microvillar actin bundles.The Journal of cell biology · 2007Article
- Apical spectrin is essential for epithelial morphogenesis but not apicobasal polarity in Drosophila.The Journal of cell biology · 1999Article
- Cellular titin localization in stress fibers and interaction with myosin II filaments in vitro.The Journal of cell biology · 1994Article
- Isolation of the bile canalicular actin-myosin II motor.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Identification and characterization of two huge protein components of the brush border cytoskeleton: evidence for a cellular isoform of titin.The Journal of cell biology · 1992Article
- Multiple polyadenylation signals and 3' untranslated sequences are conserved between chicken and human cellular myosin II transcripts.Gene expression · 1991Article
- Intestinal brush border revisited.Gut · 1989Review
- Characterization of intestinal microvillar membrane disks: detergent-resistant membrane sheets enriched in associated brush border myosin I (110K-calmodulin).The Journal of cell biology · 1989Article
- The 110-kD protein-calmodulin complex of the intestinal microvillus (brush border myosin I) is a mechanoenzyme.The Journal of cell biology · 1989Article
- Novel redistribution of myosin-containing filaments in cultured keratinocytes identified by a human monoclonal autoantibody.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1989Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We have investigated the role of myosin in contraction of the terminal web in brush borders isolated from intestinal epithelium. At 37 degrees C under conditions that stimulate terminal web contraction (1 microM Ca++ and ATP), most (60-70%) of the myosin is released from the brush border. Approximately 80% of the myosin is also released by ATP at 0 degree C, in the absence of contraction. Preextraction of this 80% of the myosin from brush borders with ATP has no effect on either the time course or extent of subsequently stimulated contraction. However, contraction is inhibited by removal of all of the myosin with 0.6 M KCl and ATP. Contraction is also inhibited by an antibody to brush border myosin, which inhibits both the ATPase activity of brush border myosin and its ability to form stable bipolar polymers. These results indicate that although functional myosin is absolutely required for terminal web contraction only approximately 20% of the brush border myosin is actually necessary. This raises the possibility that there are at least two different subsets of myosin in the terminal web.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.